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Series GSE4081 Query DataSets for GSE4081
Status Public on Mar 31, 2006
Title Expression QTL (eQTL) mapping in the anterior pituitary gland using DES-treated COPxACI F2 rats.
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). 43 COPxACI F2 animals were selected based on pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. In conjunction with genomewide genotypes available for these 43 animals, linkage analysis was done using mRNA abundance of each transcript on the array as a separate quantitative phenotype. These arrays provide a resource for expression QTL (eQTL) mapping on a genomewide level in the pituitary gland.
Keywords: Estrogen Response
 
Overall design 43 samples from anterior pituitary gland were analyzed. COPxACI F2 male animals were treated with DES for 12 weeks, starting at 9 weeks of age. Pituitary glands were harvested and weighed at sacrifice (21 weeks of age). The 43 samples were selected for pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Genomewide genotypes are available for these animals and in conjunction with the array data, genomewide expression QTL (eQTL) mapping was done.
 
Contributor(s) Deffenbacher KE, Shull JD
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Submission date Jan 23, 2006
Last update date Jul 31, 2017
Contact name James Shull
E-mail(s) shull@oncology.wisc.edu
Phone 608-265-5003
Organization name University of Wisconsin-Madison
Department McArdle Laboratory for Cancer Research
Lab Shull Laboratory
Street address 1400 University Avenue
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (43)
GSM93497 COPxACI F2 #1
GSM93498 COPxACI F2 #2
GSM93499 COPxACI F2 #3
This SubSeries is part of SuperSeries:
GSE4083 Diethylstilbestrol effects on rat anterior pituitary gland
Relations
BioProject PRJNA104581

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