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Status |
Public on Mar 31, 2006 |
Title |
Expression QTL (eQTL) mapping in the anterior pituitary gland using DES-treated COPxACI F2 rats. |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). 43 COPxACI F2 animals were selected based on pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. In conjunction with genomewide genotypes available for these 43 animals, linkage analysis was done using mRNA abundance of each transcript on the array as a separate quantitative phenotype. These arrays provide a resource for expression QTL (eQTL) mapping on a genomewide level in the pituitary gland. Keywords: Estrogen Response
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Overall design |
43 samples from anterior pituitary gland were analyzed. COPxACI F2 male animals were treated with DES for 12 weeks, starting at 9 weeks of age. Pituitary glands were harvested and weighed at sacrifice (21 weeks of age). The 43 samples were selected for pituitary weight phenotypic extremes: 22 highest and 21 lowest pituitary weights in our F2 population. Genomewide genotypes are available for these animals and in conjunction with the array data, genomewide expression QTL (eQTL) mapping was done.
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Contributor(s) |
Deffenbacher KE, Shull JD |
Citation missing |
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Submission date |
Jan 23, 2006 |
Last update date |
Jul 31, 2017 |
Contact name |
James Shull |
E-mail(s) |
shull@oncology.wisc.edu
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Phone |
608-265-5003
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Organization name |
University of Wisconsin-Madison
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Department |
McArdle Laboratory for Cancer Research
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Lab |
Shull Laboratory
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Street address |
1400 University Avenue
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platforms (1) |
GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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Samples (43)
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This SubSeries is part of SuperSeries: |
GSE4083 |
Diethylstilbestrol effects on rat anterior pituitary gland |
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Relations |
BioProject |
PRJNA104581 |