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Series GSE40737 Query DataSets for GSE40737
Status Public on Sep 11, 2012
Title Splicing switch of an epigenetic regulator by RNA helicases promotes tumor-cell invasiveness
Organism Mus musculus
Experiment type Expression profiling by array
Summary Both epigenetic and splicing regulation contribute to tumor progression, but the potential links between these two levels of gene-expression regulation in pathogenesis are not well understood. Here, we report that the mouse and human RNA helicases ddx17 and ddx5 contribute to tumor-cell invasiveness by regulating alternative splicing of several DNA- and chromatin-binding factors, including the macroH2A1 histone. We show that macroH2A1 splicing isoforms differentially regulate the transcription of a set of genes involved in redox metabolism. In particular, the SOD3 gene that encodes the extracellular superoxide dismutase and plays a part in cell migration is regulated in an inverse manner by macroH2A1 splicing isoforms. These findings reveal a new regulatory pathway in which splicing factors control the expression of histone-variant isoforms that in turn drive a transcription program to switch tumor cells to an invasive phenotype.
 
Overall design We analyzed 4T1 cells depleted or not for ddx5 and ddx17 RNA helicases using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. Four techinical replicates were performed.
We analyzed 4T1 cells depleted for ddx5 and ddx17 RNA helicases and macroH2A1.1 or macroH2A1.2 using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. Three techinical replicates were performed.
 
Contributor(s) Dardenne E, Pierredon S, Driouch K, Gratadou L, Lacroix-Triki M, Espinoza MP, Zonta E, Germann S, Mortada H, Villemin J, Dutertre M, Lidereau R, Vagner S, Auboeuf D
Citation(s) 23022728
Submission date Sep 10, 2012
Last update date Mar 06, 2018
Contact name Etienne Dardenne
E-mail(s) etienne.dardenne@gmail.com
Organization name Weill Cornell Medecine
Department Pathology
Lab Rickman
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (14)
GSM1000209 4T1 siCtrl #1
GSM1000210 4T1 siCtrl #2
GSM1000211 4T1 siCtrl #3
Relations
BioProject PRJNA174786

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40737_RAW.tar 334.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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