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| Status |
Public on Sep 09, 2012 |
| Title |
Expression data from CD34+ hematopoietic cells transduced with control or anti-HCLS1 shRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Knockdown of HCLS1 mRNA in CD34+ hematopoietic cells resulted in a severe diminished in vitro myeloid differentiation which was in line with downregulation of a set of genes, e.g., of Wnt or PI3K/Akt signaling cascades. We performed microarrays to evaluate specific genes and signaling systems regulated by HCLS1 in hematopoietic cells.
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| Overall design |
CD34+ hematopoietic cells were isolated from the bone marrow of healthy individuals, transduced with lenitvirus-based RFP-expressed shRNA constructs targeting HCLS1 mRNA (two different shRNA constructs were used simultaneously) or scrambled control shRNA. After 4 days of the transductions, RFP+ cells were sorted, and total RNA of sorted cells was isolated and used for Affymetrix Exome arrays.
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| Contributor(s) |
Skokowa J, Welte K |
| Citation(s) |
23001182 |
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| Submission date |
Sep 09, 2012 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Siarhei Kandabarau |
| E-mail(s) |
kandabarau.siarhei@mh-hannover.de
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| Organization name |
Hannover Medical School
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| Street address |
Carl-Neuberg-Str 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA174739 |
