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| Status |
Public on Sep 05, 2012 |
| Title |
Gene expression in human subcutaneous adipose tissue after administration of Gastric Insulinotropic Peptide (GIP) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The intention of this clinical study was to investigate the effect of GIP administration for 240 min on gene expression in human subcutaneous adipose tissue. Three conditions have been tersted: 1. Sole infusion of GIP or NaCl as control; 2. GIP or NaCl administration under euglycemic-hyperinsulinemic clamp conditions; 3. GIP or NaCl administration under hyperglycemic-hyperinsulinemic clamp conditions to mimic the postprandial state.
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| Overall design |
In each participant a complete physical examination and evaluation of medical history was performed, including an oral glucose tolerance test (oGTT) with 75 g glucose after overnight fast to ensure the metabolic state. Standard fasting laboratory and clinical chemistry evaluations were done. Synthetic human GIP (1-42) was dissolved in saline (0.9% NaCl-solution) under sterile conditions. All studies were done in the morning in the overnight fasted state (>10h since last meal). The effect of GIP administration on gene expression in subcutaneous adipose tissue was studied under 3 different conditions in a single blind design. Either the participants received only a GIP- or a saline- infusion (0.9% NaCl-isotonic solution, Fresenius, Germany) for 240 min. At different investigation days participants underwent euglycemic (EU)- and hyperglycemic (HC), hyperinsulinemic clamps combined with GIP- or placebo-infusions for 240 min at different examination days in a randomized, single-blind, crossover design. The capillary glucose concentration was 80mg/dl during EU-clamp and 140mg/dl during HC-clamp. The following numbers of treatments were performed: EU with GIP-infusion (N=9); EU with NaCl-infusion (N=9); HC with GIP-infusion (N=8), HC with NaCl-infusion (N=8); sole GIP-infusion (N=11) and sole placebo-infusion (N=11). Between examination days an intermission time of at least 7 days was maintained.
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| Contributor(s) |
Pfeiffer AF, Goegebakan O, Osterhoff MA, Rudovich N |
| Citation(s) |
25994074 |
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| Submission date |
Sep 04, 2012 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Martin A Osterhoff |
| Organization name |
German Institute of Human Nutrition
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| Department |
Clinical Nutrition
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| Street address |
Arthur-Scheunert-Allee 155
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| City |
Nuthetal |
| ZIP/Postal code |
14558 |
| Country |
Germany |
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| Platforms (1) |
| GPL6848 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version) |
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| Samples (96)
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| Relations |
| BioProject |
PRJNA174352 |
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