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Series GSE40589 Query DataSets for GSE40589
Status Public on Sep 05, 2012
Title Gene expression in human subcutaneous adipose tissue after administration of Gastric Insulinotropic Peptide (GIP)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The intention of this clinical study was to investigate the effect of GIP administration for 240 min on gene expression in human subcutaneous adipose tissue. Three conditions have been tersted: 1. Sole infusion of GIP or NaCl as control; 2. GIP or NaCl administration under euglycemic-hyperinsulinemic clamp conditions; 3. GIP or NaCl administration under hyperglycemic-hyperinsulinemic clamp conditions to mimic the postprandial state.
Overall design In each participant a complete physical examination and evaluation of medical history was performed, including an oral glucose tolerance test (oGTT) with 75 g glucose after overnight fast to ensure the metabolic state. Standard fasting laboratory and clinical chemistry evaluations were done. Synthetic human GIP (1-42) was dissolved in saline (0.9% NaCl-solution) under sterile conditions. All studies were done in the morning in the overnight fasted state (>10h since last meal). The effect of GIP administration on gene expression in subcutaneous adipose tissue was studied under 3 different conditions in a single blind design. Either the participants received only a GIP- or a saline- infusion (0.9% NaCl-isotonic solution, Fresenius, Germany) for 240 min. At different investigation days participants underwent euglycemic (EU)- and hyperglycemic (HC), hyperinsulinemic clamps combined with GIP- or placebo-infusions for 240 min at different examination days in a randomized, single-blind, crossover design. The capillary glucose concentration was 80mg/dl during EU-clamp and 140mg/dl during HC-clamp. The following numbers of treatments were performed: EU with GIP-infusion (N=9); EU with NaCl-infusion (N=9); HC with GIP-infusion (N=8), HC with NaCl-infusion (N=8); sole GIP-infusion (N=11) and sole placebo-infusion (N=11). Between examination days an intermission time of at least 7 days was maintained.
Contributor(s) Pfeiffer AF, Goegebakan O, Osterhoff MA, Rudovich N
Citation(s) 25994074
Submission date Sep 04, 2012
Last update date Apr 27, 2022
Contact name Martin A Osterhoff
Organization name German Institute of Human Nutrition
Department Clinical Nutrition
Street address Arthur-Scheunert-Allee 155
City Nuthetal
ZIP/Postal code 14558
Country Germany
Platforms (1)
GPL6848 Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version)
Samples (96)
GSM997377 SCAT_0_NACL_EU_2
GSM997378 SCAT_240_GIP_EU_3
GSM997379 SCAT_240_NACL_EU_4
BioProject PRJNA174352

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Supplementary file Size Download File type/resource
GSE40589_RAW.tar 474.7 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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