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| Status |
Public on Dec 05, 2012 |
| Title |
Proviral integration site for Moloney murine leukemia virus (PIM) kinases promote human T helper 1 cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of the dataset was to study on genome-wide level the effect of PIM kinase (PIM1+PIM2+PIM3) knockdown in gene expression on early differentiation of human cord blood derived CD4+ T cells cultured under Th1 (Act+IL12) polarizing conditions.
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| Overall design |
Total RNA from PIM1+PIM2+PIM3 siRNA treated cord blood CD4+ T cells 6 hours after culturing the cells in activating (antiCD3+antiCD28) plus IL-12 (Th1) or IL-4 (Th2) conditions was compared to total RNA from nonspecific control siRNA treated cells. Samples from 3 biological replicates were analysed.
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| Contributor(s) |
Tahvanainen J, Kyläniemi MK, Kanduri K, Gupta B, Lähteenmäki H, Kallonen T, Rajavuori A, Rasool O, Koskinen PJ, Rao KV, Lähdesmäki H, Lahesmaa R |
| Citation(s) |
23209281 |
| Submission date |
Aug 31, 2012 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Kartiek Kanduri |
| Phone |
+358-2-333-8001
|
| Organization name |
Turku Centre for Biotechnology
|
| Lab |
Molecular Systems Immunology / Lahesmaa
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| Street address |
Tykistokatu 6
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (12)
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| GSM995990 |
CordBlood_CD4+_control_siRNA_Act+IL12_6h__rep1 |
| GSM995991 |
CordBlood_CD4+_control_siRNA_Act+IL12_6h__rep2 |
| GSM995992 |
CordBlood_CD4+_control_siRNA_Act+IL12_6h__rep3 |
| GSM995993 |
CordBlood_CD4+_PIM123_siRNA_Act+IL12_6h__rep1 |
| GSM995994 |
CordBlood_CD4+_PIM123_siRNA_Act+IL12_6h__rep2 |
| GSM995995 |
CordBlood_CD4+_PIM123_siRNA_Act+IL12_6h__rep3 |
| GSM1036107 |
CordBlood_CD4+_control_siRNA_Act+IL4_6h__rep1 |
| GSM1036108 |
CordBlood_CD4+_control_siRNA_Act+IL4_6h__rep2 |
| GSM1036109 |
CordBlood_CD4+_control_siRNA_Act+IL4_6h__rep3 |
| GSM1036110 |
CordBlood_CD4+_PIM123_siRNA_Act+IL4_6h__rep1 |
| GSM1036111 |
CordBlood_CD4+_PIM123_siRNA_Act+IL4_6h__rep2 |
| GSM1036112 |
CordBlood_CD4+_PIM123_siRNA_Act+IL4_6h__rep3 |
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| Relations |
| BioProject |
PRJNA174342 |
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