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| Status |
Public on Sep 12, 2013 |
| Title |
Identification of Nkx6.1 regulated genes in mature pancreatic islets. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Nkx6.1 target genes were identified in mature pancreatic islets by comparing gene expression in conditional Nkx6.1-ablated islets versus control islets using microarray analysis.
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| Overall design |
Nkx6.1 was conditionally ablated in mature pancreatic islets by recombination of a Nkx6.1-flox allele using the tamoxifen-inducible Pdx1-CreERTM allele (Gu et al 2002). Mice were injected with 2 mg/25 g tamoxifen in corn oil four times between 4 and 6 weeks of age. Islets were isolated after the final tamoxifen injection. Total RNA was isolated and pooled from pancreata of 6 week old Nkx6.1fl/-;Pdx1-CreERTM (mutant) versus Nkx6.1fl/+;Pdx1-CreERTM (control) littermates for 3 biological replicates.
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| Contributor(s) |
Taylor BL, Sander MA |
| Citation(s) |
24035389 |
| |
| Submission date |
Aug 29, 2012 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Brandon Taylor |
| Organization name |
University of California, San Diego
|
| Department |
Pediatrics and Cellular & Molecular Medicine
|
| Lab |
Maike Sander, M.D.
|
| Street address |
Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Dr., Room 3101
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA174098 |
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