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Series GSE40343 Query DataSets for GSE40343
Status Public on Aug 25, 2012
Title Genome wide maps of E2F7 binding in IMR90 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Oncogene-induced senescence is an anti-proliferative stress response program that acts as a fail-safe mechanism to limit oncogenic transformation and is regulated by the retinoblastoma protein (RB) and p53 tumor suppressor pathways. We identify the atypical E2F family member E2F7 as the only E2F transcription factor potently upregulated during oncogene-induced senescence, a setting where it acts in response to p53 as a direct transcriptional target. Once induced, E2F7 binds and represses a series of E2F target genes and cooperates with RB to efficiently promote cell cycle arrest and limit oncogenic transformation. Disruption of RB triggers a further increase in E2F7, which induces a second cell cycle checkpoint that prevents unconstrained cell division despite aberrant DNA replication. Mechanistically, E2F7 compensates for the loss of RB in repressing mitotic E2F target genes.
Overall design Examination of E2F7 binding in either growing or senescent IMR90 cells with different hairpins.
Contributor(s) Aksoy O, Chicas A, Lowe SW, Wang X, Zeng T
Citation(s) 22802529
Submission date Aug 23, 2012
Last update date May 15, 2019
Contact name Ozlem Aksoy
Phone 917-930-1718
Organization name CSHL
Department Watson School of Biological Sciences
Lab Scott Lowe's lab
Street address 415 East 68th Street
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (7)
GSM991659 Growing
GSM991660 Quiescent
GSM991661 Senescent/shRen
BioProject PRJNA173795
SRA SRP015123

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Supplementary file Size Download File type/resource
GSE40343_RAW.tar 1.6 Mb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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