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Series GSE4019 Query DataSets for GSE4019
Status Public on Jan 19, 2006
Title Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803.
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary Abstract: A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation conditions and to influence the expression of a number of light-responsive genes. The nblS orthologue in Synechocystis sp. strain PCC6803 is designated dspA (also called hik33). We have generated a dspA null mutant and analyzed global gene expression in both the mutant and wild-type strains under high- and low-light conditions. The mutant is aberrant for the expression of many genes encoding proteins critical for photosynthesis, phosphate and carbon acquisition, and the amelioration of stress conditions. Furthermore, transcripts from a number of genes normally detected only during exposure of wild-type cells to high-light conditions become partially constitutive in the low-light-grown dspA mutant. Other genes for which transcripts decline upon exposure of wild-type cells to high light are already lower in the mutant during growth in low light. These results suggest that DspA may influence gene expression in both a positive and a negative manner and that the dspA mutant behaves as if it were experiencing stress conditions (e.g., high-light exposure) even when maintained at near-optimal growth conditions for wild-type cells. This is discussed with respect to the importance of DspA for regulating the responses of the cell to environmental cues.
A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species.
Keywords: strain_or_line_design
 
Overall design User Defined
 
Contributor(s) Tu C
Citation(s) 15175303
Submission date Jan 11, 2006
Last update date Mar 16, 2012
Organization Stanford Microarray Database (SMD)
E-mail array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL3356 Syn-2
Samples (20)
GSM91952 spdspA HL3hr +glucose
GSM91953 spdspA HL6hr +glucose
GSM91954 spwtGHL0.5hr
Relations
BioProject PRJNA95117

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Supplementary data files not provided

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