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| Status |
Public on Nov 30, 2012 |
| Title |
Effects of Disperse Yellow 7 in Silurana tropicalis Gene Network Analysis |
| Organism |
Xenopus tropicalis |
| Experiment type |
Expression profiling by array
|
| Summary |
Dyes used in fabric and leather industry are being released and accumulated into Canadian ecosystems. Recent studies have demonstrated that dyes made of azo compounds significantly increase toxicity in biota, which is explained by their toxic metabolites (e.g., aromatic amines). The metabolites of azo compounds interact with hydrophobic surfaces of cell membranes causing expansion of the membrane which impede normal cellular functions. It has been suggested that this process leads to cell death due to improper ion balance. Currently, it is estimated that between 10 and 15% of azo dyes are released in the environment as effluent. The aim of this study was to evaluate toxicity and gene networks altered by azo compounds in amphibians using ecotoxicogenomic approaches. Larvae of the frog Silurana tropicalis (Western clawed frog) were exposed to sediment contaminated to 887 ppm Disperse Yellow 7 (DY7). Larvae were exposed from Nieuwkoop-Faber developmental stage 12 to 46. Data suggest that the azo dye DY7 induced cellular stress and interfered with androgen biosynthesis in early tadpole development. At exposure completion, RNA was isolated from whole larvae and quality was ascertained using bioanalyzer analysis. A custom Agilent 4 X 44 K microarray for S. tropicalis was used to characterize gene regulatory networks underlying toxicity. This study presents the transcriptional regulatory pathways affected by DY7 in S. tropicalis early development.
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| Overall design |
Embryos were exposed to the DY7 dye during development. There were 4 control groups exposed and 4 treatment groups exposed.
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| Contributor(s) |
Langlois VS, Mathieu-Denoncourt J, Martyniuk CJ, de Solla S |
| Citation missing |
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| |
| Submission date |
Aug 15, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
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| Platforms (1) |
| GPL15626 |
Agilent-035843 Genotypic_EA519_Xenopus_tropicalis_GE_4x44k |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA172929 |
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