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Series GSE39994 Query DataSets for GSE39994
Status Public on Feb 11, 2013
Title TGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Purpose: We aimed to identify miRNAs which are induced by the Activin/Nodal effectors, P-Smad2/3, in order to further our understanding of how P-Smad2/3 controls downstream gene expression in mouse ES cells to regulate crucial biological processes.
Methods: We used a previously developed Tetracycline-On (Tet-On) system (TAG1) to manipulate the levels of P-Smad2/3 in mouse ES cells and performed an Illumina deep-sequencing screen to identify miRNAs which followed the P-Smad2/3 pathway.
Results: We filtered the deep-seq data to identify a list of 28 miRNAs which showed a >1.25 fold increase in response to P-Smad2/3 induction and a >1.25 fold decrease in response to P-Smad2/3 repression.
Conclusions: Our study represents a comprehensive global profiling of miRNA expression in response to changes in P-Smad2/3 levels in mouse ES cells.
Overall design miRNA profiles of TAG1 cells which were untreated (control), SB-431541 treated (P-Smad2/3 repressed), or Dox treated (P-Smad2/3 induced), were generated using Illumina GAII.
Contributor(s) Redshaw N, Camps C, Ragoussis J, Episkopou V
Citation(s) 23390484
Submission date Aug 08, 2012
Last update date May 15, 2019
Contact name Carme Camps
Organization name Wellcome Trust Centre for Human Genetics/ U. of Oxford
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX37BN
Country United Kingdom
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (3)
GSM983067 Control
GSM983068 SB
GSM983069 Dox
BioProject PRJNA172215
SRA SRP014760

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Supplementary file Size Download File type/resource
GSE39994_RAW.tar 20.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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