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| Status |
Public on Jul 05, 2013 |
| Title |
Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: PLZF_HEK] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Within the overall project, we performed a set of microarray and chromatin-immunoprecipitation (ChIP)-chip experiments using siRNA against the (pro)renin receptor ((P)RR), stable overexpression of PLZF, the PLZF translocation inhibitor genistein and the specific V-ATPase inhibitor bafilomycin to dissect transcriptional pathways downstream of the (P)RR.
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| Overall design |
In this dataset, we include the expression data obtained from stable PLZF overexpression in HEK293T cells. Three intervention samples and three control samples were analyzed. We generated the following pairwise comparisons using Chipinspector (Genomatix Software GmbH) and a FDR of 1%.
ChipInspector carries out significance analysis on the single probe level. Normalized probe set level data not provided for individual Sample records. Processed data is available on Series record.
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| Contributor(s) |
Funke-Kaiser H, Zaade D |
| Citation(s) |
23469216 |
| |
| Submission date |
Aug 08, 2012 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Daniela Zaade |
| E-mail(s) |
Daniela.Zaade@charite.de, Daniela.Zaade@gmx.de
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| Phone |
0049(0)30450525304
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| Organization name |
Charité
|
| Department |
Center for Cardiovascular Research
|
| Lab |
AG Heiko Funke-Kaiser
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| Street address |
Hessische Straße 3-4
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| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
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| Platforms (1) |
| GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (6)
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| GSM982314 |
HEK293T, stable PLZF overexpression, replica 1 |
| GSM982315 |
HEK293T, stable transfection of insertless vector, replica 1 |
| GSM982316 |
HEK293T, stable PLZF overexpression, replica 2 |
| GSM982317 |
HEK293T, stable transfection of insertless vector, replica 2 |
| GSM982318 |
HEK293T, stable PLZF overexpression, replica 3 |
| GSM982319 |
HEK293T, stable transfection of insertless vector, replica 3 |
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| This SubSeries is part of SuperSeries: |
| GSE39965 |
Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses |
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| Relations |
| BioProject |
PRJNA172169 |
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