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| Status |
Public on Jun 20, 2015 |
| Title |
The RALA pathway can maintain the proliferation of KRAS- and BRAF-mutated cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
By silencing of RALA, a downstream member of the RAS signal transduction pathway, we aimed to determine whether genes downstream of a mutated KRAS (codon 12 or 13) or a mutated BRAF can have significant functions in colorectal cancer carcinogenesis.
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| |
|
| Overall design |
RALA was silenced in three colorectal cancer cell lines (SW480, HCT116 and HT29). Effects were normalized to mock-transfected cells and the effects of scramble siRNA were excluded.
SW480, HCT116 and HT29 cell lines were treated with the PI3K inhibitor LY294002 or DMSO.
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| |
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| Contributor(s) |
Gyorffy B, Stelniec-Klotz I, Schäfer R |
| Citation(s) |
26033452 |
| |
| Submission date |
Aug 02, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Balazs Györffy |
| E-mail(s) |
zsalab2@yahoo.com
|
| Organization name |
Hungarian Academy of Sciences
|
| Street address |
Bókay 53
|
| City |
Budapest |
| ZIP/Postal code |
H1083 |
| Country |
Hungary |
| |
|
| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (15)
|
| GSM980430 |
SW480 cells treated with transfection reagents only |
| GSM980431 |
SW480 cells treated with Scrambled siRNA (nonsense sequence) |
| GSM980432 |
SW480 cells treated with RALA-specific siRNA |
| GSM980433 |
HCT116 cells treated with transfection reagents only |
| GSM980434 |
HCT116 cells treated with Scrambled siRNA (nonsense sequence) |
| GSM980435 |
HCT116 cells treated with RALA-specific siRNA |
| GSM980436 |
HT29 cells treated with transfection reagents only |
| GSM980437 |
HT29 cells treated with Scrambled siRNA (nonsense sequence) |
| GSM980438 |
HT29 cells treated with RALA-specific siRNA |
| GSM980439 |
HCT116 cells treated with DMSO |
| GSM980440 |
HCT116 cells treated with LY294002 |
| GSM980441 |
HT29 cells treated with DMSO |
| GSM980442 |
HT29 cells treated with LY294002 |
| GSM980443 |
SW480 cells treated with DMSO |
| GSM980444 |
SW480 cells treated with LY294002 |
|
| Relations |
| BioProject |
PRJNA171925 |
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