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Series GSE39540 Query DataSets for GSE39540
Status Public on Jul 21, 2012
Title A mesenchymal stromal cell gene signature for donor age
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human aging is associated with loss of function and regenerative capacity. Human bone marrow derived mesenchymal stromal cells (hMSCs) are involved in tissue regeneration, evidenced by their capacity to differentiate into several lineages and therefore are considered the gold standard for cell-based regeneration therapy. Tissue maintenance and regeneration is dependent on stem cells and declines with age and aging is thought to influence therapeutic efficacy, therefore, more insight in the process of aging of hMSCs is of high interest. We, therefore, hypothesized that hMSCs might reflect signs of aging. In order to find markers for donor age, early passage hMSCs were isolated from bone marrow of 61 donors, with ages varying from 17-84, and clinical parameters, in vitro characteristics and microarray analysis were assessed. Although clinical parameters and in vitro performance did not yield reliable markers for aging since large donor variations were present, genome-wide microarray analysis resulted in a considerable list of genes correlating with human age. By comparing the transcriptional profile of aging in human with the one from rat, we discovered follistatin as a common marker for aging in both species. The gene signature presented here could be a useful tool for drug testing to rejuvenate hMSCs or for the selection of more potent, hMSCs for cell-based therapy.
We used microaray gene expression profiling to find expressed genes that correlated with the donor age of bone-marrow derived mesenchymal stromal cells (hMSCs)
 
Overall design Human mesenchymal stem cells (hMSC) were biopsied from bone marrow from 61 different donors aging from 17 - 89 years old. The genome wide expression profiles was assessed using Affymetrix microarrays. The expression of genes was correlated with the age of the donors.
 
Contributor(s) Alves H, van Ginkel J, Groen N, Hulsman M, Mentink A, Reinders M, van Blitterswijk C, de Boer J
Citation(s) 22927939
Submission date Jul 20, 2012
Last update date Dec 06, 2018
Contact name Nathalie Groen
E-mail(s) n.groen@utwente.nl
Phone 0031534893062
Organization name University of Twente - MIRA – Institute for Biomedical Technology and Technical Medicine
Department Tissue Regeration
Street address Drienerlolaan 5
City Enschede
ZIP/Postal code 7522 NB
Country Netherlands
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (61)
GSM971351 hMSC - Acetabulum - 28F (013914_h)
GSM971352 hMSC - Acetabulum - 29F (013915_h)
GSM971353 hMSC - Iliac crest - 84F (013916_h)
Relations
BioProject PRJNA171065

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39540_RAW.tar 188.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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