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Series GSE39459 Query DataSets for GSE39459
Status Public on Nov 30, 2012
Title Genome-wide maps of PRC2 complex components and chromatin states in prostate cancer cell lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the high-throughput profiling of PRC2 core subunits chromatin states and histone modifications in two prostate cancer cell lines, androgen-dependent cells LNCaP and androgen-independent cells LNCaP-abl (abl). By obtaining over 1 billion bases of sequence from chromatin immunoprecipitated DNA, we generated the genome-wide localizations of EZH2, SUZ12 and H3K27 trimethylation in both cell lines, and found that EZH2 can be localized to a subsets of chromating sites that don't have H3K27me3 or SUZ12 signals nearby. Interestingly, these sites are enriched for the active histone marks H3K4 di/trimethylation as well as the RNA polymerase II, which suggest the potential function of these peaks in gene activation. Indeed, these sites are enriched near the transcription start sites of EZH2-activated genes. Further analysis of the transcription factor motifs enriched at these peaks revealed the enrichement of androgen receptor motif, suggesting a co-activator role for EZH2 in concert with AR. Our work demonstrated a novel funtion of EZH2 in transcriptional activation by directly binding to the chromatin sites that cooperate with AR.
Overall design Study of the chromatin localizations of PRC2 complex core subunits and different histone marks in 2 cell types
Contributor(s) Xu K, Wu ZJ
Citation(s) 23239736
Submission date Jul 18, 2012
Last update date May 15, 2019
Contact name Kexin Xu
Organization name UT Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl, MC 8257
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (12)
GSM969562 EZH2_ChIPSeq_abl
GSM969563 H3K27me3_ChIPSeq_abl
GSM969564 SUZ12_ChIPSeq_abl
This SubSeries is part of SuperSeries:
GSE39461 Role of PRC2 complex components in prostate cancer cell lines
BioProject PRJNA170921
SRA SRP014457

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Supplementary file Size Download File type/resource
GSE39459_RAW.tar 1.7 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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