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Series GSE39411 Query DataSets for GSE39411
Status Public on Jan 04, 2013
Title Expression data from healthy and malignant (chronic lymphocytic leukemia, CLL) human B-lymphocytes after B-cell receptor stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Three different cell populations (6 healthy B-lymphocytes, 6 leukemic CLL B-lymphocyte of indolent form and 5 leukemic CLL B-lymphocyte of aggressive form) were stimulated in vitro with an anti-IgM antibody, activating the B-cell receptor (BCR). We analyzed the gene expression at 4 time points (60, 90, 210 and 390 minutes). Each gene expression measurement is performed both in stimulated cells and in control unstimulated cells.
For one aggressive CLL case, we silenced expression of DUSP1 by transfecting DUSP1-specific RNAi and, as a control, transfected cells with a non-targeting RNAi. We then stimulated the BCR of these cells and analyzed the gene expression at the same time points in stimulated cells and in control unstimulated cells.
 
Overall design B-cells were negatively selected from healthy donors and previously untreated CLL patients. BCR stimulated and unstimulated control B-cells were treated at four time points after stimulation for total RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Gribben JG, Vallat L
Citation(s) 23267079
Submission date Jul 17, 2012
Last update date Mar 25, 2019
Contact name Laurent Vallat
E-mail vallat@unistra.fr
Organization name Strasbourg University Hospital
Department Immunology
Street address 1, Place de l'Hopital
City Strasbourg
ZIP/Postal code 67091
Country France
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (152)
GSM967665 Healthy B-cell, subject N1_unstimulated cells_at 60min
GSM967666 Healthy B-cell, subject N1_unstimulated cells_at 90min
GSM967667 Healthy B-cell, subject N1_unstimulated cells_at 210min
Relations
BioProject PRJNA170836

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Supplementary file Size Download File type/resource
GSE39411_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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