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| Status |
Public on May 09, 2023 |
| Title |
Expression data of human induced pluripotent stem cells (hiPSCs), human embryonic stem cells (hESCs) and those differentiated cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Third-party reanalysis
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| Summary |
We examined hiPSCs, hESCs and those differentiated cells to identify pluripotent signature genes, differentiation marker genes and relationship between expression and phenotypes.
Additionally, we performed microarray experiments to examine gene expression in human tissues. This data was used for comparison with hESCs, hiPSCs and those differentiated cells.
A total of 22 tissues (bone marrow, cerebellum, colon, cortex, fetal brain, heart, kidney, liver, lung, pancreas, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testes, thymus, thyroid, trachea and uterus) were examined.
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| Overall design |
The hiPSCs, hESCs and those differentiated cells were cultured, and their cDNAs were used for microarray analysis.
Total RNA isolated from cultivated cells and human tissues were labeled and hybridized to the GeneChip Human Genome U133 Plus 2.0 Array according to the manufacturer's protocol.
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| Contributor(s) |
Okada Y, Miya F, Koike M, Tomisato S, Tokura T, Ishihara Y, Shimojo D, Kanematsu D, Kanemura Y, Kohda K, Sobue G, Yamanaka S, Yuzaki M, Uchiyama Y, Ikeda E, Tsunoda T, Okano H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 05, 2012 |
| Last update date |
May 10, 2023 |
| Contact name |
Fuyuki Miya |
| E-mail(s) |
fmiya@keio.jp
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| Phone |
+81-3-5363-3890
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| Organization name |
Keio University
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| Department |
School of Medicine
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| Lab |
Center for Medical Genetics
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| Street address |
35 Shinanomachi, Shinjuku-ku
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| City |
Tokyo |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (77)
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| Relations |
| BioProject |
PRJNA170115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39144_RAW.tar |
552.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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