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Series GSE39005 Query DataSets for GSE39005
Status Public on Mar 01, 2013
Title FS001: Aberrant gene expression in dogs with portosystemic shunts
Organism Canis lupus familiaris
Experiment type Expression profiling by array
Summary Background: Congenital portosystemic shunts are developmental anomalies of the vascular system that cause portal blood to bypass the liver. Large-breed dogs are reported to have a predisposition for intrahepatic portosystemic shunts (IHPSS) and small-breed dogs a predisposition for extrahepatic portosystemic shunts (EHPSS). While the physiological consequences of the two types of shunt are identical, the metabolic pathways involved are probably different. The aim of this study was to gain insight into the metabolic pathways involved in the different types of portosystemic shunting. Results: Microarray analysis of mRNA expression in liver tissue from dogs with EHPSS and IHPSS revealed that the expression of 26 genes was altered in either IHPSS or EHPSS samples compared with that in liver samples from control dogs. Quantitative real-time PCR of these genes in 14 IHPSS, 17 EHPSS, and 8 control liver samples revealed a significantly different expression of ACBP, CCBL1, GPC3, HAMP, PALLD, VCAM1, and WEE1. Immunohistochemistry studies revealed that the pattern of VCAM1 and WEE1 protein expression supports the involvement of these proteins in the development of EHPSS and IHPSS, respectively. Conclusions: We identified a small list of genes possibly involved in two genetically different types of portosystemic shunt with identical pathophysiological consequences. WEE1 was found to be aberrantly expressed at an RNA and protein level in dogs with IHPSS. A decreased VCAM1 expression may play a role in the development of intrahepatic portal vascularization in dogs with EHPSS.
Overall design Total RNA was isolated from liver tissue from 2 healthy dogs, 32 dogs with EHPSS, and 14 dogs with IHPSS. Pooled RNA isolated from healthy liver tissue was used as reference. Dye swap of Cy3 and Cy5 was performed to reduce dye bias.
Contributor(s) van Steenbeek FG, van den Bossche L, Grinwis GC, Kummeling A, van Gils IH, Groot Koerkamp MJ, van Leenen D, Holstege FC, Penning L, Rothuizen J, Leegwater PA, Spee B
Citation(s) 23451256, 23472125, 24945279, 29770973, 29049355
Submission date Jun 28, 2012
Last update date Jul 28, 2019
Contact name Marian Groot Koerkamp
Organization name UMC Utrecht, Netherlands
Department Molecular Cancer Research
Lab Holstegelab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3508 AB
Country Netherlands
Platforms (1)
GPL15184 A-UMCU-C44K-1.0
Samples (92)
GSM953602 FS001-225613 vs. FS001-ref
GSM953603 FS001-227889 vs. FS001-ref
GSM953604 FS001-301714 vs. FS001-ref
BioProject PRJNA169652

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39005_RAW.tar 177.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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