NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE38997 Query DataSets for GSE38997
Status Public on Oct 01, 2012
Title Expression data from Caenorhabditis elegans L1-stage larvae exposed to ethidium bromide and/or serial ultraviolet C radiation
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Ultraviolet C radiation (UVC) damages the nuclear and mitochondrial genomes; this damage is repaired in the nuclear but not mitochondrial genome. Ethidium bromide (EtBr) inhibits mitochondrial DNA replication.
We were interested in the transcriptomic response to exposure to UVC, EtBr, and the combination. The UVC exposure protocol results in a high level of mitochondrial DNA damage, and a low level of nuclear DNA damage (because of repair).
 
Overall design We exposed age-matched L1-stage Caenorhabditis elegans to ultraviolet C radiation (UVC ) three times, separated in time by 24 h, in the absence of food. After the third exposure, larvae were placed on K agar plates with OP50 bacterial food. In some cases ethidium bromide was also used. Nematodes were sampled for RNA isolation several times.
 
Contributor(s) Meyer J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 28, 2012
Last update date Jul 06, 2016
Contact name Joel Meyer
E-mail(s) joel.meyer@duke.edu
Organization name Duke University
Street address Box 90328 LSRC A354
City Durham
State/province NC
ZIP/Postal code 27708-0328
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (69)
GSM953405 experiment 1 control samples at 48 h 1c48
GSM953406 experiment 1 UVC-exposed samples at 48 h 1u48
GSM953407 experiment 1 EtBr-exposed samples at 48 h 1e48
Relations
BioProject PRJNA169554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38997_RAW.tar 133.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap