 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 07, 2012 |
| Title |
HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Heat-Shock Factor 1 (HSF1), master regulator of the heat-shock response, facilitates malignant transformation, cancer cell survival and proliferation in model systems. The common assumption is that these effects are mediated through regulation of heat-shock protein (HSP) expression. However, the transcriptional network that HSF1 coordinates directly in malignancy and its relationship to the heat-shock response have never been defined. By comparing cells with high and low malignant potential alongside their non-transformed counterparts, we identify an HSF1-regulated transcriptional program specific to highly malignant cells and distinct from heat shock. Cancer-specific genes in this program support oncogenic processes: cell-cycle regulation, signaling, metabolism, adhesion and translation. HSP genes are integral to this program, however, even these genes are uniquely regulated in malignancy. This HSF1 cancer program is active in breast, colon and lung tumors isolated directly from human patients and is strongly associated with metastasis and death. Thus, HSF1 rewires the transcriptome in tumorigenesis, with prognostic and therapeutic implications.
|
| |
|
| Overall design |
ChIP-seq was used to characterize HSF1 binding
|
| |
|
| Contributor(s) |
Mendillo ML, Santagata S, Koeva M, Bell GW, Hu R, Tamimi RM, Fraenkel E, Ince TA, Whitesell L, Lindquist S |
| Citation(s) |
22863008 |
| |
| Submission date |
Jun 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Marc Mendillo |
| Organization name |
Whitehead Institute for Biomedical Research
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
|
| Samples (63)
|
|
| This SubSeries is part of SuperSeries: |
| GSE38912 |
HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers |
|
| Relations |
| SRA |
SRP013883 |
| BioProject |
PRJNA169333 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38901_Table_S1.xls.gz |
2.1 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...