|
| Status |
Public on Jun 29, 2012 |
| Title |
Expression Data from siRae1 and siNT infected with VSV or mock infected |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To test the effect of silencing Rae1 on expression on RNA polymerase II transcripts, host mRNAs were analysed by cDNA microarrays. We hypothesized that if silencing Rae1 expression increases cellular resistance to inhibition of transcription in VSV infected cells, mRNA characteristic of host antiviral response would be increased than compared to cells transfected with control siRNA.
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| Overall design |
HeLa cells transfected with siRNA targeted against Rae1 (siRae1) or against non-targeting siRNA (siNT). The sequence of nontargeting (NT) siRNA is scrambled and does not match any sequence on the human genome. Transfected cells were either infected with VSV or mock infected. Six hours later RNA was extracted from the cells. Two separate experiments of such were analyzed by microarrays
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| |
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| Contributor(s) |
Rajani K, Lyles DS, Chou JW |
| Citation(s) |
23028327 |
| Submission date |
Jun 21, 2012 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Jeff Chou |
| E-mail(s) |
jchou@wakehealth.edu
|
| Phone |
3367572617
|
| Organization name |
Atrium Health Wake Forest Baptist
|
| Street address |
Medical Center Blvd
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA169081 |
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