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| Status |
Public on Jan 17, 2013 |
| Title |
Expression data from Saccharomyces cerevisiae |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study we focus on two Saccharomyces cerevisiae strains with varying production of heterologous α-amylase and we compare the metabolic fluxes and transcriptional regulation at aerobic and anaerobic conditions, in particular with the objective to identify the final electron acceptor for protein folding.
We found that anaerobic conditions showed high amount of amylase productions when comparing to aerobic conditions and the genome-scale transcriptional analysis suggested that genes related to the endoplasmic reticulum (ER), lipid synthesis and stress responses were generally up-regulated at anaerobic conditions. Moreover, we proposed a model for the electron transfer from ER to the final electron acceptor, fumarate under anaerobic conditions.
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| Overall design |
Three Saccharomyces cerevisiae strains with varied amylase productions were selected at early glucose phase in batch fermentations for RNA extraction and hybridization on Affymetrix microarrays. Biological triplicates were applied, and strains with empty plasmid (no amylase productions) were used as control strain.
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| Contributor(s) |
Liu Z, Österlund T |
| Citation(s) |
23435897 |
| Submission date |
Jun 21, 2012 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Tobias Österlund |
| E-mail(s) |
tobiaso@chalmers.se
|
| Organization name |
Chalmers University of Technology
|
| Department |
Dept. of Chemical and Biological Engineering
|
| Lab |
Systems biology
|
| Street address |
Kemivägen 10
|
| City |
Göteborg |
| ZIP/Postal code |
41296 |
| Country |
Sweden |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (18)
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| GSM950692 |
AAC strain_aerobic conditions_biological rep1 |
| GSM950693 |
AAC strain_aerobic conditions_biological rep2 |
| GSM950694 |
AAC strain_aerobic conditions_biological rep3 |
| GSM950695 |
AAP strain_aerobic conditions_biological rep1 |
| GSM950696 |
AAP strain_aerobic conditions_biological rep2 |
| GSM950697 |
AAP strain_aerobic conditions_biological rep3 |
| GSM950698 |
NC strain_aerobic conditions_biological rep1 |
| GSM950699 |
NC strain_aerobic conditions_biological rep2 |
| GSM950700 |
NC strain_aerobic conditions_biological rep3 |
| GSM950701 |
AAC strain_anaerobic conditions_biological rep1 |
| GSM950702 |
AAC strain_anaerobic conditions_biological rep2 |
| GSM950703 |
AAC strain_anaerobic conditions_biological rep3 |
| GSM950704 |
AAP strain_anaerobic conditions_biological rep1 |
| GSM950705 |
AAP strain_anaerobic conditions_biological rep2 |
| GSM950706 |
AAP strain_anaerobic conditions_biological rep3 |
| GSM950707 |
NC strain_anaerobic conditions_biological rep1 |
| GSM950708 |
NC strain_anaerobic conditions_biological rep2 |
| GSM950709 |
NC strain_anaerobic conditions_biological rep3 |
|
| Relations |
| BioProject |
PRJNA169097 |
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