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| Status |
Public on Jan 01, 2013 |
| Title |
Histone deacetylation at coding sequences adjusts transcription kinetics during Candida albicans morphogenesis [ChIP-seq] |
| Organism |
Candida albicans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The dataset contains ChIP-Seq data of the Set3 and Hos2 proteins in Candida albicans, assayed in two morphological phases (yeast and hypha). The Set3 and Hos2 proteins in the respective strains carry 9myc epitopes and ChIP was performed with an anti-myc antibody. Included samples are the following: 1 input and 1 ChIP sample of an untagged wild type strain as negative control assayed in the yeast phase, 1 input and 3 ChIP biological replicates of the Set3-9myc strain in the yeast phase, 1 input and 2 ChIP biological replicates of the Set3-9myc strain in the hypha phase, 1 input and 2 ChIP biological replicates of the Hos2-9myc strain in the yeast phase, 1 input and 2 ChIP biological replicates of the Hos2-9myc strain in the hypha phase, 1 input and 3 ChIP biological replicates of Set3-9myc in a set1delta/delta background in the yeast phase.
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| Overall design |
ChIP-Seq was performed of Candida albicans strains in two morphological phases (yeast and hypha). Yeast-phase cells were grown to the exponential phase in YPD at 30C. Hyphal differentiation was induced by resuspending the cells in YPD+20% Fetal Calf Serum and a shift of the growth temperature to 37C. Induction was performed for 30 minutes. Cells were crosslinked with 1% formaldehyde for 15 minutes at room temperature.
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| Contributor(s) |
Hnisz D |
| Citation(s) |
23236295 |
| |
| Submission date |
Jun 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Denes Hnisz |
| E-mail(s) |
dhnisz@yahoo.at, denes.hnisz@meduniwien.ac.at
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| Organization name |
Max F Perutz Laboratories
|
| Department |
Medical Biochemistry
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| Lab |
Kuchler
|
| Street address |
Dr Bohr-gasse
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL15149 |
Illumina Genome Analyzer IIx (Candida albicans) |
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| Samples (19)
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| This SubSeries is part of SuperSeries: |
| GSE38427 |
Histone deacetylation at coding sequences adjusts transcription kinetics during Candida albicans morphogenesis. |
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| Relations |
| BioProject |
PRJNA167841 |
| SRA |
SRP013497 |
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