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Status |
Public on Jul 04, 2013 |
Title |
Identification of Nrf2-regulated genes in A549 lung cancer cells by oligonucleotide microarray |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To elucidate the mechanisms by which Nrf2 regulates cell growth, we performed global gene expression profiling of A549 lung cancer cells with knockdown of Nrf2. Gene networks associated with carbohydrate metabolism and drug metabolism were significantly downregulated in Nrf2-depleted A549 cells. Gene Set Enrichment Analysis revealed significant enrichment of genes associated with carbohydrate catabolic processes, positive regulation of metabolic processes, PPP, and arachidonic acid metabolism. In summary, this analysis revealed that Nrf2 positively regulates transcription of genes that play key roles in central carbon metabolism.
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Overall design |
A549 cells were transfected with non targeting NS siRNA or siRNA targeting Nrf2. Mock transfected A549 cells were treated with transfection reagent alone. We had 3 biological replicates for each of the 3 groups. Ninty six hours post transfection, cells were lysed and total RNA was isolated.
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Contributor(s) |
Singh A, Acquaah-Mensah G, Biswal S |
Citation(s) |
23921124 |
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Submission date |
May 30, 2012 |
Last update date |
Mar 25, 2019 |
Contact name |
George Acquaah-Mensah |
E-mail(s) |
george.acquaah-mensah@mcphs.edu
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Phone |
5083735643
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Organization name |
MASS COLLEGE OF PHARM HEALTH SCIENCES
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Street address |
19 Foster Street
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City |
Worcester |
State/province |
MA |
ZIP/Postal code |
01608 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (9)
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Relations |
BioProject |
PRJNA167681 |