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Series GSE38332 Query DataSets for GSE38332
Status Public on Jul 04, 2013
Title Identification of Nrf2-regulated genes in A549 lung cancer cells by oligonucleotide microarray
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To elucidate the mechanisms by which Nrf2 regulates cell growth, we performed global gene expression profiling of A549 lung cancer cells with knockdown of Nrf2. Gene networks associated with carbohydrate metabolism and drug metabolism were significantly downregulated in Nrf2-depleted A549 cells. Gene Set Enrichment Analysis revealed significant enrichment of genes associated with carbohydrate catabolic processes, positive regulation of metabolic processes, PPP, and arachidonic acid metabolism. In summary, this analysis revealed that Nrf2 positively regulates transcription of genes that play key roles in central carbon metabolism.
Overall design A549 cells were transfected with non targeting NS siRNA or siRNA targeting Nrf2. Mock transfected A549 cells were treated with transfection reagent alone. We had 3 biological replicates for each of the 3 groups. Ninty six hours post transfection, cells were lysed and total RNA was isolated.
Contributor(s) Singh A, Acquaah-Mensah G, Biswal S
Citation(s) 23921124
Submission date May 30, 2012
Last update date Mar 25, 2019
Contact name George Acquaah-Mensah
Phone 5083735643
Street address 19 Foster Street
City Worcester
State/province MA
ZIP/Postal code 01608
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (9)
GSM940245 A549_Mock_biological rep1
GSM940246 A549_Mock_biological rep2
GSM940247 A549_Mock_biological rep3
BioProject PRJNA167681

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Supplementary file Size Download File type/resource
GSE38332_RAW.tar 50.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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