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Status |
Public on Nov 05, 2012 |
Title |
Genome-wide mapping of FOXM1 binding sites |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
There are nearly fifty forkhead (FOX) transcription factors encoded in the human genome and due to sharing a common DNA binding domain, they are all thought to bind to similar DNA sequences. It is therefore unclear how these transcription factors are targeted to specific chromatin regions to elicit specific biological effects. Here we used ChIP-seq to investigate the genome-wide chromatin binding mechanisms used by the forkhead transcription factor FOXM1. In keeping with its previous association with cell cycle control, we demonstrate that FOXM1 binds and regulates a group of genes which are mainly involved in controlling late cell cycle events in the G2 and M phases. However, rather than being recruited through canonical RYAAAYA forkhead binding motifs, FOXM1 binding is directed via CHR elements. FOXM1 binds these elements through protein-protein interactions with the MMB transcriptional activator complex. Thus, we have uncovered a novel and unexpected mode of chromatin binding of a FOX transcription factor which allows it to specifically control cell cycle-dependent gene expression.
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Overall design |
Examination of FOXM1 binding sites in G2/M U2OS cells
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Contributor(s) |
Chen X, Sharrocks AD |
Citation(s) |
23109430 |
Submission date |
May 23, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Xi Chen |
E-mail(s) |
Xi.Chen@manchester.ac.uk
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Organization name |
The University of Manchester
|
Department |
Faculty of Life Sciences
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Lab |
Sharrocks Lab
|
Street address |
Michael Smith Building, Dover Street
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City |
Manchester |
ZIP/Postal code |
M13 9PT |
Country |
United Kingdom |
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Platforms (1) |
GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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Samples (3) |
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Relations |
SRA |
SRP013345 |
BioProject |
PRJNA167358 |