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Series GSE38170 Query DataSets for GSE38170
Status Public on Nov 05, 2012
Title Genome-wide mapping of FOXM1 binding sites
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary There are nearly fifty forkhead (FOX) transcription factors encoded in the human genome and due to sharing a common DNA binding domain, they are all thought to bind to similar DNA sequences. It is therefore unclear how these transcription factors are targeted to specific chromatin regions to elicit specific biological effects. Here we used ChIP-seq to investigate the genome-wide chromatin binding mechanisms used by the forkhead transcription factor FOXM1. In keeping with its previous association with cell cycle control, we demonstrate that FOXM1 binds and regulates a group of genes which are mainly involved in controlling late cell cycle events in the G2 and M phases. However, rather than being recruited through canonical RYAAAYA forkhead binding motifs, FOXM1 binding is directed via CHR elements. FOXM1 binds these elements through protein-protein interactions with the MMB transcriptional activator complex. Thus, we have uncovered a novel and unexpected mode of chromatin binding of a FOX transcription factor which allows it to specifically control cell cycle-dependent gene expression.
 
Overall design Examination of FOXM1 binding sites in G2/M U2OS cells
 
Contributor(s) Chen X, Sharrocks AD
Citation(s) 23109430
Submission date May 23, 2012
Last update date May 15, 2019
Contact name Xi Chen
E-mail(s) Xi.Chen@manchester.ac.uk
Organization name The University of Manchester
Department Faculty of Life Sciences
Lab Sharrocks Lab
Street address Michael Smith Building, Dover Street
City Manchester
ZIP/Postal code M13 9PT
Country United Kingdom
 
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (3)
GSM936206 FOXM1_ChIP-seq_rep1
GSM936207 FOXM1_ChIP-seq_rep2
GSM936208 Input_DNA
Relations
SRA SRP013345
BioProject PRJNA167358

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38170_FOXM1_peak_summits.bed.gz 2.7 Kb (ftp)(http) BED
GSE38170_RAW.tar 673.5 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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