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Status |
Public on Jul 16, 2012 |
Title |
sibling aHDF-iPSC clones derived from the same fibroblasts |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, little attention has so far been paid to variations among hiPSC lines in terms of their hepatic differentiation. We developed an improved hepatic differentiation protocol and compared multiple hiPSC lines. This comparison indicated that the hepatic differentiation propensity varies among sibling hiPSC clones derived from the same adult human dermal fibroblasts (aHDFs). In addition, hiPSC clones derived from peripheral blood cells (PB-iPSCs) consistently showed good hepatic differentiation efficiency, whereas many hiPSC clones from adult dermal fibroblasts (aHDF-iPSCs) showed poor hepatic differentiation. However, when we compared hiPSCs from blood and dermal fibroblasts from the same individuals, we found that variations in hepatic differentiation were largely attributable to donor differences, rather than to the types of the original cells. In order to understand the molecular mechanisms underlying the observed variations in hepatic differentiation, we performed microarray analyses of sibling aHDF-iPSC clones, and aHDF- and PB-iPSC clones from the same individuals.
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Overall design |
sibling aHDF-iPSC clones (201B6 and 201B7; derived from the same aHDFs) (1) undifferentiated state (n=4, biological replicate #1-#4) (2) CXCR4-positive cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4) (3) CXCR4-negative cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4)
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Contributor(s) |
Kajiwara M, Narita M |
Citation(s) |
22802639 |
Submission date |
May 23, 2012 |
Last update date |
Oct 11, 2016 |
Contact name |
Shinya Yamanaka |
E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
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Phone |
81-75-366-7041
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Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
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Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
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City |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
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Platforms (1) |
GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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Samples (24)
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This SubSeries is part of SuperSeries: |
GSE38155 |
Donor-dependent hepatic differentiation propensity among hiPSC lines. |
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Relations |
BioProject |
PRJNA167463 |