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| Status |
Public on May 30, 2012 |
| Title |
Hepatic gene expression in streptozotocin-induced diabetic mice fed a phloridzin diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Phloridzin is a dihydrochalcone typically contained in apples. A diet containing 0.5 % phloridzin significantly improves hyperglycemia but not hypoinsulinemia and tissue lipid peroxidation in streptozotocin (STZ)-induced diabetic mice after 14 days. The phloridzin diet has no effect on the alteration of hepatic gene expression in STZ-induced diabetic mice.
A quantitative RT-PCR analysis showed a reversal of the STZ induction of the sodium/glucose cotransporter gene Sglt1 and the drug-metabolizing enzyme genes Cyp2b10 and Ephx1 in the small intestine of mice fed a 0.5% phloridzin diet. These mice also showed a reversal of the STZ-mediated renal induction of the glucose-regulated facilitated glucose transporter gene Glut2. Dietary phloridzin improved the abnormal elevations in blood glucose level and the overexpression of Sglt1, Cyp2b10 and Ephx1 in the small intestine of STZ-induced diabetic mice.
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| Overall design |
Six-week-old male mice were divided into 4 groups of 6 mice each, housed in groups of 3 per cage. After 1 week mice were intraperitoneally injected with STZ. Mice (n=6) in the untreated control group did not receive any treatment. After 1 week, 18 mice showing non-fasting blood glucose levels of 330-590 mg/dL were divided into 3 groups: one group was fed with AIN93G only (control group), the others with an AIN93G diet containing 0.1% or 0.5% phloridzin (Funakoshi, Tokyo, Japan) for 2 weeks.
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| Contributor(s) |
Kobori M |
| Citation(s) |
19413312 |
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| Submission date |
May 22, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Masuko Kobori |
| E-mail(s) |
kobori@affrc.go.jp
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| Organization name |
Food Research Institute, National Agriculture and Food Research Organization
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| Street address |
2-1-13 Kannondai
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8642 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA167276 |
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