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| Status |
Public on May 23, 2012 |
| Title |
Aspergillus nidulans WT, ΔcrzA and ΔcrzA/scrA-: Control vs. calcium pulse |
| Organism |
Aspergillus nidulans |
| Experiment type |
Expression profiling by array
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| Summary |
The main objective was to identifiy genes related to scrA- extragenic suppression of crzA. Thus, through transcriptional profiling, we characterised the transcriptional basis of calcium tolerance in our mutant strain, ΔcrzA/scrA-. To evaluate the effect of calcium on global A. nidulans gene expression, we performed competitive microarray hybridizations using RNA obtained from the wild-type, ΔcrzA and ΔcrzA/scrA- strains before and after short pulses (10 and 30 minutes) of 200 mM calcium chloride. The experiment was further validated by real-time PCR.
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| Overall design |
Two-condition experiment : A. nidulans wild-type, ΔcrzA and ΔcrzA/scrA- strains with 200 mM CaCl2 for 10 and 30 min at 30°C and 150 rpm in 25 mL YAG medium. Firstly, the strains were grown overnight at 30°C and 150 rpm in YAG medium (preculture). Afterwards, the medium was switched by a new media (25 mL) plus calcium and incubated as described above. Control condition refers to the preculture in YAG medium without further incubation.
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| Contributor(s) |
Almeida RS, Savoldi M, de Souza WR, Goldman GH |
| Citation missing |
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| Submission date |
May 22, 2012 |
| Last update date |
May 24, 2012 |
| Contact name |
Wagner Rodrigo de Souza |
| E-mail(s) |
wagnerusp@gmail.com
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| Phone |
+55 16 3602 4311
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| Organization name |
University of Sao Paulo
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| Department |
Pharmaceutical Sciences
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| Lab |
Molecular Biology of Microrganisms
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| Street address |
Av do Cafe
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| City |
Ribeirao Preto |
| State/province |
Sao Paulo |
| ZIP/Postal code |
14040903 |
| Country |
Brazil |
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| Platforms (1) |
| GPL15593 |
Agilent_029535 Univ. of Sao Paulo-FCFRP/Mol Biol Lab Aspergillus nidulans 4X44K v.1.0 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA167342 |
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