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| Status |
Public on Apr 24, 2012 |
| Title |
Transcriptomic Responses of Synechocystis sp. PCC 6803 Encapsulated in Silica Gel |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
|
| Summary |
Global gene expression of Synechocystis sp. PCC 6803 encapsulated in silica gel was examined by microarray analysis. Cultures were encapsulated in gels derived from aqueous precursors and gels derived from alkoxide precursors and incubated under constant light for 24 hours prior to RNA extraction. Cultures suspended in liquid media were also exposed to 500 mM salt stress and incubated under identical conditions, for comparison purposes. The expression of 414 genes was significantly altered by encapsulation in aqueous-derived gels (fold change >/= 1.5 and P-value < 0.01), the expression of 1143 genes were significantly altered by encapsulation in alkoxide derived gels, and only 243 genes were common to both encapsulation chemistries. Additional qRT-PCR analyses of four select genes; ggpS, cpcG2, slr5055, and sll5057, confirmed microarray results. These results illustrate that encapsulation stress is quite different than salt stress in terms of gene expression response. Furthermore, a number of hypothetical and unknown proteins associated with encapsulation and alcohol stress have been identified, with implications for improving encapsulation protocols and rationally engineering microorganisms for direct biofuel production.
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| Overall design |
16 samples; 4 biological replicates each of 4 treatments
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| |
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| Contributor(s) |
Luterra MD, Dickson DJ, Ely RL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 23, 2012 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Markael D. Luterra |
| E-mail |
luterram@engr.orst.edu
|
| Phone |
651-307-5945
|
| Organization name |
Oregon State University
|
| Department |
Biological and Ecological Engineering
|
| Lab |
Roger Ely, Ph.D.
|
| Street address |
116 Gilmore Hall
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97333 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15471 |
NimbleGen Synechocystis sp. PCC 6803 Gene Expression Array [101018_TI1148_60mer_exp] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA161507 |
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