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Series GSE37482 Query DataSets for GSE37482
Status Public on Apr 24, 2012
Title Transcriptomic Responses of Synechocystis sp. PCC 6803 Encapsulated in Silica Gel
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary Global gene expression of Synechocystis sp. PCC 6803 encapsulated in silica gel was examined by microarray analysis. Cultures were encapsulated in gels derived from aqueous precursors and gels derived from alkoxide precursors and incubated under constant light for 24 hours prior to RNA extraction. Cultures suspended in liquid media were also exposed to 500 mM salt stress and incubated under identical conditions, for comparison purposes. The expression of 414 genes was significantly altered by encapsulation in aqueous-derived gels (fold change >/= 1.5 and P-value < 0.01), the expression of 1143 genes were significantly altered by encapsulation in alkoxide derived gels, and only 243 genes were common to both encapsulation chemistries. Additional qRT-PCR analyses of four select genes; ggpS, cpcG2, slr5055, and sll5057, confirmed microarray results. These results illustrate that encapsulation stress is quite different than salt stress in terms of gene expression response. Furthermore, a number of hypothetical and unknown proteins associated with encapsulation and alcohol stress have been identified, with implications for improving encapsulation protocols and rationally engineering microorganisms for direct biofuel production.
 
Overall design 16 samples; 4 biological replicates each of 4 treatments
 
Contributor(s) Luterra MD, Dickson DJ, Ely RL
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Submission date Apr 23, 2012
Last update date Apr 25, 2012
Contact name Markael D. Luterra
E-mail luterram@engr.orst.edu
Phone 651-307-5945
Organization name Oregon State University
Department Biological and Ecological Engineering
Lab Roger Ely, Ph.D.
Street address 116 Gilmore Hall
City Corvallis
State/province OR
ZIP/Postal code 97333
Country USA
 
Platforms (1)
GPL15471 NimbleGen Synechocystis sp. PCC 6803 Gene Expression Array [101018_TI1148_60mer_exp]
Samples (16)
GSM920860 Control-rep1
GSM920861 Control-rep2
GSM920862 Control-rep3
Relations
BioProject PRJNA161507

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37482_RAW.tar 25.0 Mb (http)(custom) TAR (of PAIR)
Raw data provided as supplementary file
Processed data included within Sample table

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