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Series GSE37432 Query DataSets for GSE37432
Status Public on Jun 18, 2012
Title Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA)
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary piRNAs are required to maintain germline integrity and fertility but their mechanism of action is poorly understood. Here we demonstrate that C. elegans piRNAs silence transcripts in trans through imperfectly complementary sites. We find that target silencing is independent of Piwi endonuclease activity or “slicing”. Instead, we show that piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene, pseudogene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes but not pseudogenes or transposons. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNAi in C. elegans.
 
Overall design Affymetrix mRNA expression data from wild-type and two independent prg-1;prg-2 double mutant C. elegans strains (mRNA)
 
Contributor(s) Bagijn MP, Goldstein LD, Sapetschnig A, Weick E, Bouasker S, Lehrbach NJ, Simard MJ, Miska EA
Citation(s) 22700655, 22738725
Submission date Apr 19, 2012
Last update date Jul 06, 2016
Contact name Leonard Goldstein
Organization name Genentech, Inc.
Street address 1 DNA Way
City South San Francisco
ZIP/Postal code 94080
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (9)
GSM919250 N2 rep1
GSM919251 N2 rep2
GSM919252 N2 rep3
This SubSeries is part of SuperSeries:
GSE37433 Function, targets and evolution of Caenorhabditis elegans piRNAs
Relations
BioProject PRJNA160201

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37432_RAW.tar 18.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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