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Series GSE3720 Query DataSets for GSE3720
Status Public on Feb 28, 2006
Title Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The two major human gd T cell subsets, Vd1 and Vd2, display differences in tissue tropism and agonist responses, but we have little insight into global differences that may exist at the gene expression level. This is due to the small numbers of these cells that can be obtained from healthy donors, which limit comprehensive, comparative gene expression analyses. We established a culture method that expands Vd1 and Vd2 cells from the same PBL preparation to levels sufficient for sorting and microarray analysis. Although the subsets were expanded identically (anti-TCR mAb, plus IL-15), 392 and 614 genes were identified, which were differentially expressed in the two subsets, from two donors, respectively. Approximately 4,500 genes changed in both subsets following PMA/ionomycin treatment; about 50% of these genes were subset-specific. Both subsets responded to a crude LPS preparation, but only 6% of the responsive genes were the same. The differentially expressed genes were consistent with Vd2 cells being more inflammatory and Vd1 cells having more of a regulatory phenotype. Both subsets expressed transcripts encoding an array of innate and NK cell receptors, supporting the relationship of gd?T cells to the innate immune system. Our results show that circulating Vd1 and Vd2 subsets in humans have considerable, inherent differences in gene expression following treatment with non-TCR agonists, supporting unique functional roles for these cells in vivo.
Keywords: cell type comparison
 
Overall design To analyze global gene expression differences between Vd1 and Vd2 gd T cells, 12 Affymetrix Human Genome U133A 2.0 chips were run. 2 replicate sets of 6 chips from 2 donors were run; each set of six chips consisted of 3 chips for Vd1 cells and 3 chips for Vd2 cells. The 3 chips for each subset consisted of cells treated with PBS, LPS, or PMA/ionomycin for 4 hours just prior to RNA extraction.
 
Contributor(s) Kress E, Hedges JF, Jutila MA
Citation(s) 16423401
Submission date Dec 02, 2005
Last update date Dec 06, 2018
Contact name Ellen Kress
E-mail ekress@montana.edu
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM85804 Vd1 PBS rep1
GSM85805 Vd1 PBS rep2
GSM85806 Vd1 LPS rep1
Relations
BioProject PRJNA93873

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