|
| Status |
Public on Jul 26, 2012 |
| Title |
HITS-CLIP analysis of FUS in lineage-committed cells. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In the present study, we performed HITS-CLIP analysis for FUS using mouse brain to extensively characterize tits RNA-binding sites and functional roles in RNA metabolisms. We identified preferential binding of FUS to stem-and-loop structures but without any discernible consensus motifs. FUS was preferentially bound to introns and 3' untranslated regions, but the exon/intron boundaries were mostly devoid of FUS-tags. Analysis of position-dependence of FUS-binding sites in regulating inclusion and skipping of exons disclosed that FUS is bound broadly around the alternatively spliced exons. Among them, however, noticeable CLIP-tags were observed in the downstream introns. We also noticed that FUS occasionally binds to the antisense strands in the promoter regions. Global analysis of CLIP-tags and expression profiles revealed that binding of FUS to the promoter antisense regions downgregulates transcription of the sense strand.
|
| |
|
| Overall design |
HITS-CLIP (High Throughput Sequencing after Crosslinking and Immunoprecipitation) experiments targeting FUS in mouse cerebrums derived from 12-week-old C57BL/6 mice
|
| |
|
| Contributor(s) |
Masuda A, Ohno K |
| Citation(s) |
22829983 |
| |
| Submission date |
Apr 11, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Akio Masuda |
| E-mail(s) |
amasuda@med.nagoya-u.ac.jp
|
| Phone |
+81-52-744-2447
|
| Organization name |
Nagoya University Graduate School of Medicine
|
| Lab |
Neurogenetics
|
| Street address |
65 tsurumai-cho, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL9318 |
AB SOLiD System 3.0 (Mus musculus) |
|
| Samples (1) |
|
| Relations |
| SRA |
SRP012157 |
| BioProject |
PRJNA158981 |
