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Series GSE3719 Query DataSets for GSE3719
Status Public on Dec 07, 2005
Title knock-out Slr1738 mutant strain analysis exposed to cadmium (50 microM).
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The transcriptome approach was used to characterize the global change in slr1738 null-mutant gene expression in response to cadmium (50 microM, 180 min), compared to wild type strain gene expression in response to cadmium (50 microM, 180 min). Total RNA were isolated from null-mutant and wild type stressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: stress responses
 
Overall design Our analysis (slr1738 null-mutant strain Cd VS wild type strain Cd) is composed of 2 arrays (dye swap).
 
Contributor(s) Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F
Citation(s) 17910763
Submission date Dec 02, 2005
Last update date Nov 12, 2013
Contact name Franck Chauvat
E-mail franck.chauvat@cea.fr
Phone 33 1 69 08 78 11
Organization name CEA Saclay
Department DSV/DBJC
Lab LSOC
Street address -
City Gif sur yvette CEDEX
ZIP/Postal code 91191
Country France
 
Platforms (1)
GPL3214 Takara CyanoChip v.2.0
Samples (2)
GSM85682 slr1738 null mutant cadmium (CdSO4 50 (M 180 min), replicate 1
GSM85683 slr1738 null mutant cadmium (CdSO4 50 (M 180 min), replicate 2
This SubSeries is part of SuperSeries:
GSE3755 Synechocystis PCC6803 Stress Response
Relations
BioProject PRJNA94303

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