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Series GSE3717 Query DataSets for GSE3717
Status Public on Dec 07, 2005
Title Synechocystis PCC6803 dose responses to iron starvation (96h).
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The transcriptome approach was used to characterize the global change (2 Fe concentrations) in Synechocystis gene expression in response to continuous iron starvation, compared to standard medium (17 microM). For iron depletion, exponentially growing cells were washed in Fe-free medium, and grown for 48 hrs under standard conditions in liquid medium cotaining either 1 mM or 2 mM of ferric ammonium citrate. Then, cells were washed, and resuspended in Fe-free medium (Fe contaminations 0.5 microM) and grown for another 48 hrs period. For each concentration point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each concentration point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: stress responses
Overall design We made 2 concentration points of iron starvation (2 to 0.5 microM, or 1 to 0.5 microM). For each concentration point, we analyzed 2 arrays (dye swap).
Contributor(s) Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F
Citation(s) 17910763
Submission date Dec 02, 2005
Last update date Nov 12, 2013
Contact name Franck Chauvat
Phone 33 1 69 08 78 11
Organization name CEA Saclay
Department DSV/DBJC
Street address -
City Gif sur yvette CEDEX
ZIP/Postal code 91191
Country France
Platforms (1)
GPL3214 Takara CyanoChip v.2.0
Samples (4)
GSM85678 Synechocystis cells grown in an iron depleted medium (2-0.5 (M) 96h , replicate 1
GSM85679 Synechocystis cells grown in an iron depleted medium (2-0.5 (M) 96h , replicate 2
GSM85680 Synechocystis cells grown in an iron depleted medium (1-0.5 (M) 96h , replicate 1
This SubSeries is part of SuperSeries:
GSE3755 Synechocystis PCC6803 Stress Response
BioProject PRJNA94299

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