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| Status |
Public on Dec 07, 2005 |
| Title |
Synechocystis PCC6803 time course responses to zinc (ZnSO4, 776 microM. |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcriptome approach was used to characterize the of the global change (2 time points) in Synechocystis gene expression in response to continuous exposure to zinc excess (776 microM), compared to standard medium (776 nM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: stress responses
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| Overall design |
We made 2 time points of exposure to zinc (776 microM). For each time point, we analyzed 2 arrays (dye swap).
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| Contributor(s) |
Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F |
| Citation(s) |
17910763 |
| Submission date |
Dec 02, 2005 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Franck Chauvat |
| E-mail |
franck.chauvat@cea.fr
|
| Phone |
33 1 69 08 78 11
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| Organization name |
CEA Saclay
|
| Department |
DSV/DBJC
|
| Lab |
LSOC
|
| Street address |
-
|
| City |
Gif sur yvette CEDEX |
| ZIP/Postal code |
91191 |
| Country |
France |
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| Platforms (1) |
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| Samples (4)
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| GSM85670 |
Synechocystis cells treated 240 min with zinc 776 (M (ZnSO4), replicate 1 |
| GSM85671 |
Synechocystis cells treated 240 min with zinc 776 (M (ZnSO4), replicate 2 |
| GSM85672 |
Synechocystis cells treated 30 min with zinc 776 (M (ZnSO4), replicate 1 |
| GSM85673 |
Synechocystis cells treated 30 min with zinc 776 (M (ZnSO4), replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE3755 |
Synechocystis PCC6803 Stress Response |
|
| Relations |
| BioProject |
PRJNA94297 |
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