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Series GSE3716 Query DataSets for GSE3716
Status Public on Dec 07, 2005
Title Synechocystis PCC6803 time course responses to zinc (ZnSO4, 776 microM.
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The transcriptome approach was used to characterize the of the global change (2 time points) in Synechocystis gene expression in response to continuous exposure to zinc excess (776 microM), compared to standard medium (776 nM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: stress responses
 
Overall design We made 2 time points of exposure to zinc (776 microM). For each time point, we analyzed 2 arrays (dye swap).
 
Contributor(s) Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F
Citation(s) 17910763
Submission date Dec 02, 2005
Last update date Nov 12, 2013
Contact name Franck Chauvat
E-mail franck.chauvat@cea.fr
Phone 33 1 69 08 78 11
Organization name CEA Saclay
Department DSV/DBJC
Lab LSOC
Street address -
City Gif sur yvette CEDEX
ZIP/Postal code 91191
Country France
 
Platforms (1)
GPL3214 Takara CyanoChip v.2.0
Samples (4)
GSM85670 Synechocystis cells treated 240 min with zinc 776 (M (ZnSO4), replicate 1
GSM85671 Synechocystis cells treated 240 min with zinc 776 (M (ZnSO4), replicate 2
GSM85672 Synechocystis cells treated 30 min with zinc 776 (M (ZnSO4), replicate 1
This SubSeries is part of SuperSeries:
GSE3755 Synechocystis PCC6803 Stress Response
Relations
BioProject PRJNA94297

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