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| Status |
Public on Sep 10, 2013 |
| Title |
Expression data from Escherichia coli O157:H7, strain EDL933, cultivated in caecal content of rats associated with the human intestinal microbiota |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli O157:H7 str. EDL933 |
| Experiment type |
Expression profiling by array
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| Summary |
The human intestinal microbiota associated with rats produces in vivo a soluble(s) factor(s) that down-regulates the expression of genes encoding for the Shiga toxin II in E. coli O157:H7. The Shiga toxin II is one of the major virulence factors of E. coli enterohemorragic leading to the deadly hemolitic and uremic syndrome. Investigation of the effect of the human intestinal microbiota on the whole transcriptome of EHEC O157:H7 is of major importance to increase our understanding of the pathogen transcriptomic adaptation in response to the human microbiota.
We analysed by microarray hybridization the gene expression pattern of EHEC O157:H7 grown in the caecal content of germ-free rats or rats associated with the human microbiota of a healthy human subject. By doing so, we increased our understanding of the regulatory activities of the human gut microbiota on E. coli O157:H7
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| Overall design |
A first group of twelve weeks old, male, germfree rats was colonized with the human fecal microbiota and a second group was kept germfree and condidered as a controle group. Rats were fed for two weeks with a sterile human type diet, and were sacrificed. E. coli O157:H7 was cultivated for 6 hours in the caecal content of germfree rats and rats associated with the human intestinal microbiota. RNAs were extracted and cDNAs were synthesized, fragmented and biotinylated before being hybridized on Affymetrix E. coli genome 2.0 arrays. The effect of the human intestinal microbiota was investigated by comparing the gene expression level in the caecal content of rats associated with the human microbiota with their expression level in the caecal content of the germfree rats.
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| Contributor(s) |
Le Bihan G, Garneau P, Del'homme C, Jubelin G, Martin C, Harel J |
| Citation missing |
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| Submission date |
Apr 08, 2012 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Guillaume Le Bihan |
| Organization name |
GREMIP, UdM
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| Street address |
3200 Sicotte
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| City |
St-Hyacinthe |
| State/province |
Québec |
| ZIP/Postal code |
J2S 2M2 |
| Country |
Canada |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (6)
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| GSM910630 |
Germfree rat's caecum, biological rep1 |
| GSM910631 |
Germfree rat's caecum, biological rep2 |
| GSM910632 |
Germfree rat's caecum, biological rep3 |
| GSM910633 |
Human intestinal microbiota associated rat's caecum, biological rep1 |
| GSM910634 |
Human intestinal microbiota associated rat's caecum, biological rep2 |
| GSM910635 |
Human intestinal microbiota associated rat's caecum, biological rep3 |
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| Relations |
| BioProject |
PRJNA158139 |
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