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Series GSE3703 Query DataSets for GSE3703
Status Public on Dec 07, 2005
Title Synechocystis PCC6803 time course responses to H2O2 3mM.
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The transcriptome approach was used to characterize the kinetics (5 time points) of the global change in Synechocystis gene expression in response to continuous exposure to H2O2 (3 mM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: time course, stress responses
Overall design We made a kinetic (5 time points) of exposure to H2O2 (3 mM). For each time point, we analyzed 2 arrays (dye swap).
Contributor(s) Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F
Citation(s) 17910763
Submission date Nov 30, 2005
Last update date Nov 12, 2013
Contact name Franck Chauvat
Phone 33 1 69 08 78 11
Organization name CEA Saclay
Department DSV/DBJC
Street address -
City Gif sur yvette CEDEX
ZIP/Postal code 91191
Country France
Platforms (1)
GPL3214 Takara CyanoChip v.2.0
Samples (10)
GSM85528 Synechocystis cells treated 180 min with 3mM H2O2, replicate 1
GSM85529 Synechocystis cells treated 180 min with 3mM H2O2, replicate 2
GSM85530 Synechocystis cells treated 420 min with 3mM H2O2, replicate 1
This SubSeries is part of SuperSeries:
GSE3755 Synechocystis PCC6803 Stress Response
BioProject PRJNA94289

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