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| Status |
Public on Dec 07, 2005 |
| Title |
Synechocystis PCC6803 time course responses to H2O2 3mM. |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
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| Summary |
The transcriptome approach was used to characterize the kinetics (5 time points) of the global change in Synechocystis gene expression in response to continuous exposure to H2O2 (3 mM). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: time course, stress responses
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| Overall design |
We made a kinetic (5 time points) of exposure to H2O2 (3 mM). For each time point, we analyzed 2 arrays (dye swap).
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| Contributor(s) |
Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F |
| Citation(s) |
17910763 |
| Submission date |
Nov 30, 2005 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Franck Chauvat |
| E-mail(s) |
franck.chauvat@cea.fr
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| Phone |
33 1 69 08 78 11
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| Organization name |
CEA Saclay
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| Department |
DSV/DBJC
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| Lab |
LSOC
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| Street address |
-
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| City |
Gif sur yvette CEDEX |
| ZIP/Postal code |
91191 |
| Country |
France |
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| Platforms (1) |
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| Samples (10)
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| GSM85528 |
Synechocystis cells treated 180 min with 3mM H2O2, replicate 1 |
| GSM85529 |
Synechocystis cells treated 180 min with 3mM H2O2, replicate 2 |
| GSM85530 |
Synechocystis cells treated 420 min with 3mM H2O2, replicate 1 |
| GSM85531 |
Synechocystis cells treated 420 min with 3mM H2O2, replicate 2 |
| GSM85532 |
Synechocystis cells treated 300 min with 3mM H2O2, replicate 1 |
| GSM85533 |
Synechocystis cells treated 300 min with 3mM H2O2, replicate 2 |
| GSM85534 |
Synechocystis cells treated 30 min with 3mM H2O2, replicate 1 |
| GSM85535 |
Synechocystis cells treated 30 min with 3mM H2O2, replicate 2 |
| GSM85536 |
Synechocystis cells treated 15 min with 3mM H2O2, replicate 1 |
| GSM85537 |
Synechocystis cells treated 15 min with 3mM H2O2, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE3755 |
Synechocystis PCC6803 Stress Response |
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| Relations |
| BioProject |
PRJNA94289 |
| Supplementary data files not provided |
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