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Series GSE36985 Query DataSets for GSE36985
Status Public on Oct 05, 2012
Title Comparative profiling of chromatin state maps and transcription factor occupancy during human fetal and adult erythropoiesis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the comparative investigation of genome-wide chromatin state maps, transcription factor (TF) occupancy, and gene expression profiles from developing red cell precursors at two developmental stages. Contrasting the similarities and differences between fetal and adult erythropoiesis provides important insights into the erythroid gene expression programs and gene regulatory networks. Specifically, comparative analyses of human erythropoiesis identify developmental stage-specific enhancers as primary determinants of stage-specific gene expression programs. We find that master regulators, such as GATA1 and TAL1, cooperatively act within active enhancers but have little predictive value for stage-specific enhancer activity. Instead, a set of stage-specific co-regulators collaborates with master regulators and contributes to differential gene expression. We further identify and validate IRF2, IRF6, and MYB as effectors of adult-stage expression program. Thus, the combinatorial assembly of master regulators and transcriptional co-regulators at developmental stage-specific enhancers controls gene expression programs and temporal regulation of transcriptional networks in a mammalian genome.
Overall design Examination of various histone modifications and transcription factor occupancy by ChIP-seq in fetal and adult proerythroblasts.
Contributor(s) Xu J, Shao Z, Orkin SH
Citation(s) 23041383
Submission date Apr 02, 2012
Last update date May 15, 2019
Contact name Jian Xu
Phone 214-648-6125
Organization name UT Southwestern Medical Center
Department Children's Research Institute
Street address 6000 Harry Hines Blvd. NL12.138B
City Dallas
State/province TX
ZIP/Postal code 75235
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL14761 Helicos HeliScope (Homo sapiens)
Samples (37)
GSM908034 H3K4me1-F
GSM908035 H3K4me1-A
GSM908036 H3K4me2-F
This SubSeries is part of SuperSeries:
GSE36994 Comparative profiling of human fetal and adult erythropoiesis
BioProject PRJNA157469
SRA SRP012028

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Supplementary file Size Download File type/resource
GSE36985_RAW.tar 11.3 Gb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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