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Series GSE36940 Query DataSets for GSE36940
Status Public on Jan 17, 2014
Title Cross-Species Comparison Identifies Proneural specific patterns of genetic alterations in Glioblastoma
Organism Mus musculus
Experiment type Genome variation profiling by genome tiling array
Summary The determinants of the genetic complexity of Glioblastoma are poorly understood. We generated murine Glioblastomas by transforming glial progenitors in the adult brain with PDGF expression and PTEN deletion +/- p53 deletion. PDGF+PTEN-/- tumors developed additional deletions of specific genes in up to 100% of the tumors, whereas PDGF+PTEN-/-p53-/- tumors did not. Cross-species comparison with data from tCGA database and published in Verhaak, 2010, showed that consistent genetic deletions observed in mouse tumors were specific to human Proneural Glioblastoma. These findings show that the genetic alterations that accumulate during tumor progression are determined by the initiating genetic alterations and by the cellular context in which they occur.
 
Overall design Murine gliomas were induced in vivo by retroviral mediated PDGF overexpression, PTEN deletion with or without p53 deletion using Cre/lox system. Tumors were subsequently harvested for sequencing and aCGH analysis. Paired liver DNA was used for hybridization. For PDGF+PTEN-/- tumors, different timepoints were obtained including 21, 35 days post tumor induction, as well as endstage tumors.
 
Contributor(s) Sonabend A
Citation(s) 24390738
Submission date Mar 29, 2012
Last update date Jan 17, 2014
Contact name Richard Leung
E-mail(s) rl2533@columbia.edu
Organization name Columbia University
Department Department of Neurosurgery
Lab Bartoli Brain Tumor Lab
Street address 1130 Saint Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10031
Country USA
 
Platforms (1)
GPL15076 Agilent-014695 Mouse Genome CGH Microarray 244A (G4415A)(Probe Name version)
Samples (31)
GSM906693 pTEN Deleted Endstage, replicate 1
GSM906694 pTEN Deleted Endstage, replicate 2
GSM906695 pTEN Deleted Endstage, replicate 3
Relations
BioProject PRJNA157131

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36940_LogRatiosByProbe_MedianWithin_NotSegmented.txt.gz 36.2 Mb (ftp)(http) TXT
GSE36940_RAW.tar 2.1 Gb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data are available on Series record

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