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Series GSE36888 Query DataSets for GSE36888
Status Public on Apr 25, 2012
Title Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function (RNA)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cytokine-activated STAT proteins dimerize and bind to high-affinity motifs, and N-terminal domain-mediated oligomerization of dimers allows tetramer formation and binding to low-affinity tandem motifs, but the functions of dimers versus tetramers are unknown. We generated Stat5a and Stat5b double knock-in (DKI) N-domain mutant mice that form dimers but not tetramers, identified cytokine-regulated genes whose expression required STAT5 tetramers, and defined consensus motifs for dimers versus tetramers. Whereas Stat5- deficient mice exhibited perinatal lethality, DKI mice were viable, indicating that STAT5 dimers were sufficient for survival. Nevertheless, STAT5 DKI mice had fewer CD4+CD25+ T cells, NK cells, and CD8+ T cells, with impaired cytokine-induced proliferation and homeostatic proliferation of CD8+ T cells. DKI CD8+ T cell proliferation following viral infection was diminished and DKI Treg cells did not efficiently control colitis. Thus, tetramerization of STAT5 is dispensable for survival but is critical for cytokine responses and normal immune function.
 
Overall design T cells were extracted from spleen of wt and STAT5 double knocked in mice, and treated with IL-2. The cells were collected from 0h (without treatment), 2h, 6h and 17h, and chipped on Affy mouse 430 2.0 arrays.
 
Contributor(s) Lin J, Li P, Liu D, T Jin H, He J, Rasheed M, Rochman Y, Wang L, Cui K, Liu C, Kelsall BL, Ahmed R, Leonard WJ
Citation(s) 22520852
Submission date Mar 28, 2012
Last update date Feb 11, 2019
Contact name Peng Li
E-mail(s) peng.li@nih.gov
Organization name NIH
Department NHLBI
Lab LMI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (38)
GSM904849 STAT5 double knocked in control 0 h rep 1
GSM904850 STAT5 double knocked in control 0 h rep 2
GSM904851 STAT5 double knocked in control 0 h rep 3
This SubSeries is part of SuperSeries:
GSE36890 Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function
Relations
BioProject PRJNA157371

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36888_RAW.tar 138.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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