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Series GSE36833 Query DataSets for GSE36833
Status Public on Mar 28, 2012
Title Gene Expression profiling in DBA/2J glaucoma
Organism Mus musculus
Experiment type Expression profiling by array
Third-party reanalysis
Summary Glaucoma is a common ocular disorder that is a leading cause of blindness worldwide. It is characterized by the dysfunction and loss of retinal ganglion cells (RGCs). Although many studies have implicated various molecules in glaucoma, no mechanism has been shown to be responsible for the earliest detectable damage to RGCs and their axons in the optic nerve. Here, we show that the leukocyte transendothelial migration pathway is activated in the optic nerve head at the earliest stages of disease in an inherited mouse model of glaucoma. This resulted in proinflammatory monocytes entering the optic nerve prior to detectable neuronal damage. A 1-time x-ray treatment prevented monocyte entry and subsequent glaucomatous damage. A single x-ray treatment of an individual eye in young mice provided that eye with long-term protection from glaucoma but had no effect on the contralateral eye. Localized radiation treatment prevented detectable neuronal damage and dysfunction in treated eyes, despite the continued presence of other glaucomatous stresses and signaling pathways. Injection of endothelin-2, a damaging mediator produced by the monocytes, into irradiated eyes, combined with the other glaucomatous stresses, restored neural damage with a topography characteristic of glaucoma. Together, these data support a model of glaucomatous damage involving monocyte entry into the optic nerve. Genome-wide assessment of gene expression changes was performed in DBA/2J-Gpnmb+, DBA/2J mice and irradiated DBA/2J mice at 8.5 and 10.5 months of age.
Overall design In this study (Howell et al, JCI, 2012), 50 samples (10 D2-Gpnmb+ control at 8.5 mos, 20 NOE DBA/2J at 8.5 mos, 10 radiation-treated DBA2J at 8.5 mos and 10 radiation-treated DBA/2J at 10.5 mos) were combined with 30 of ONH samples from the GSE26299 study (10 D2-Gpnmb+ and 20 NOE DBA/2J all at 10.5 mos). One D2-Gpnmb+ 8.5mo sample failed QC and was not included in the analysis. Quantile normalization was performed for all optic nerve head samples reported in the study. The complete dataset is linked below as a supplementary file.
Contributor(s) Howell GR, John SW
Citation(s) 22426214
Submission date Mar 27, 2012
Last update date Feb 11, 2019
Contact name Gareth Rhys Howell
Phone 207 288 6572
Fax 207 288 6078
Organization name The Jackson Laboratory
Street address 600 Main Street
City Mount Desert
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (49)
GSM898147 Rad-D2 rep C4.7 (ONH)
GSM898148 Rad-D2 rep C3.6 (ONH)
GSM898149 Rad-D2 rep C4.3 (ONH)
Reanalysis of GSM685892
Reanalysis of GSM685893
Reanalysis of GSM685894
Reanalysis of GSM685895
Reanalysis of GSM685896
Reanalysis of GSM685897
Reanalysis of GSM685898
Reanalysis of GSM685899
Reanalysis of GSM685900
Reanalysis of GSM685901
Reanalysis of GSM685902
Reanalysis of GSM685903
Reanalysis of GSM685904
Reanalysis of GSM685905
Reanalysis of GSM685906
Reanalysis of GSM685907
Reanalysis of GSM685908
Reanalysis of GSM685909
Reanalysis of GSM685910
Reanalysis of GSM685911
Reanalysis of GSM685942
Reanalysis of GSM685943
Reanalysis of GSM685944
Reanalysis of GSM685945
Reanalysis of GSM685946
Reanalysis of GSM685947
Reanalysis of GSM685948
Reanalysis of GSM685949
Reanalysis of GSM685950
Reanalysis of GSM685951
BioProject PRJNA156985

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36833_RAW.tar 169.7 Mb (http)(custom) TAR (of CEL)
GSE36833_complete_dataset.txt.gz 17.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data is available on Series record
Raw data provided as supplementary file

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