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| Status |
Public on Dec 01, 2005 |
| Title |
Synechocystis PCC6803 time course responses to 50 µM cadmium |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
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| Summary |
The transcriptome approach was used to characterize the kinetics (9 time points) of the global change in Synechocystis gene expression in response to continuous exposure to Cd (CdSO4, 50 _M). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point), covering 2,845 of the 3,168 ORFs of Synechocystis (Custom-commercial array : IntelliGeneTM CyanoCHIP version 1.2, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: time course, stress responses
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| Overall design |
We made a kinetic (9 time points) of exposure to cadmium (CdSO4, 50 _M). For each time point, we analyzed 2 arrays (dye swap).
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| Contributor(s) |
Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F |
| Citation(s) |
17910763 |
| Submission date |
Nov 28, 2005 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Franck Chauvat |
| E-mail(s) |
franck.chauvat@cea.fr
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| Phone |
33 1 69 08 78 11
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| Organization name |
CEA Saclay
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| Department |
DSV/DBJC
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| Lab |
LSOC
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| Street address |
-
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| City |
Gif sur yvette CEDEX |
| ZIP/Postal code |
91191 |
| Country |
France |
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| Platforms (1) |
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| Samples (18)
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| GSM84561 |
Synechocystis cells treated 15 min with 50 µM cadmium sulfate, replicate 1 |
| GSM84562 |
Synechocystis cells treated 15 min with 50 µM cadmium sulfate, replicate 2 |
| GSM84563 |
Synechocystis cells treated 180 min with 50 µM cadmium sulfate, replicate 1 |
| GSM84564 |
Synechocystis cells treated 180 min with 50 µM cadmium sulfate, replicate 2 |
| GSM84995 |
Synechocystis cells treated 300 min with 50 µM cadmium sulfate, replicate 1 |
| GSM84996 |
Synechocystis cells treated 300 min with 50 µM cadmium sulfate, replicate 2 |
| GSM84997 |
Synechocystis cells treated 30 min with 50 µM cadmium sulfate, replicate 1 |
| GSM84998 |
Synechocystis cells treated 30 min with 50 µM cadmium sulfate, replicate 2 |
| GSM84999 |
Synechocystis cells treated 90 min with 50 µM cadmium sulfate, replicate 1 |
| GSM85000 |
Synechocystis cells treated 90 min with 50 µM cadmium sulfate, replicate 2 |
| GSM85001 |
Synechocystis cells treated 360 min with 50 µM cadmium sulfate, replicate 1 |
| GSM85002 |
Synechocystis cells treated 360 min with 50 µM cadmium sulfate, replicate 2 |
| GSM85003 |
Synechocystis cells treated 960 min with 50 µM cadmium sulfate, replicate 1 |
| GSM85004 |
Synechocystis cells treated 960 min with 50 µM cadmium sulfate, replicate 2 |
| GSM85237 |
Synechocystis cells treated 75 min with 50 µM cadmium sulfate, replicate 1 |
| GSM85238 |
Synechocystis cells treated 75 min with 50 µM cadmium sulfate, replicate 2 |
| GSM85239 |
Synechocystis cells treated 60 min with 50 µM cadmium sulfate, replicate 1 |
| GSM85240 |
Synechocystis cells treated 60 min with 50 µM cadmium sulfate, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE3755 |
Synechocystis PCC6803 Stress Response |
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| Relations |
| BioProject |
PRJNA94287 |
| Supplementary data files not provided |
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