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Series GSE3682 Query DataSets for GSE3682
Status Public on Dec 01, 2005
Title Synechocystis PCC6803 time course responses to 50 µM cadmium
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The transcriptome approach was used to characterize the kinetics (9 time points) of the global change in Synechocystis gene expression in response to continuous exposure to Cd (CdSO4, 50 _M). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point), covering 2,845 of the 3,168 ORFs of Synechocystis (Custom-commercial array : IntelliGeneTM CyanoCHIP version 1.2, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays.
Keywords: time course, stress responses
 
Overall design We made a kinetic (9 time points) of exposure to cadmium (CdSO4, 50 _M). For each time point, we analyzed 2 arrays (dye swap).
 
Contributor(s) Houot L, Floutier M, Marteyn B, Legrain P, Cassier-Chauvat C, Chauvat F
Citation(s) 17910763
Submission date Nov 28, 2005
Last update date Nov 12, 2013
Contact name Franck Chauvat
E-mail franck.chauvat@cea.fr
Phone 33 1 69 08 78 11
Organization name CEA Saclay
Department DSV/DBJC
Lab LSOC
Street address -
City Gif sur yvette CEDEX
ZIP/Postal code 91191
Country France
 
Platforms (1)
GPL3095 Takara CyanoChip v.1.2
Samples (18)
GSM84561 Synechocystis cells treated 15 min with 50 µM cadmium sulfate, replicate 1
GSM84562 Synechocystis cells treated 15 min with 50 µM cadmium sulfate, replicate 2
GSM84563 Synechocystis cells treated 180 min with 50 µM cadmium sulfate, replicate 1
This SubSeries is part of SuperSeries:
GSE3755 Synechocystis PCC6803 Stress Response
Relations
BioProject PRJNA94287

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