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| Status |
Public on Nov 26, 2005 |
| Title |
Beta-naphthoflavone treated worms |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
The soil nematode Caenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its genome contains 80 cytochrome P450 genes (CYP). In order to study CYP gene expression in C. elegans mixed stages and synchronized hermaphrodites were exposed to 18 known xenobiotic cytochrome P450 inducers. Messenger RNA expression was detected by DNA arrays and semiquantitative RT-PCR. Using subfamily-specific primers, a pooled set of exon-rich CYP fragments could be amplified. In this way it was possible to systematically check the influence of different inducers on CYP expression at the same time. The well-known CYP1A inducers beta-naphthoflavone, PCB52, and lansoprazol were the most active and in particular they strongly induced almost all CYP35 isoforms. A few number of further CYP forms were found to be inducible by other xenobiotics like phenobarbital, atrazine, and clofibrate. In addition, a transgenic C. elegans line expressing GFP under control of the CYP35A2 promoter showed a strong induction of the fusion by beta-naphthoflavone in the intestine. Copyright 2001 Academic Press.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
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| Overall design |
Computed
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| Contributor(s) |
Menzel R |
| Citation(s) |
11697852 |
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| Submission date |
Nov 25, 2005 |
| Last update date |
Mar 16, 2012 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
array@genome.stanford.edu
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| Phone |
650-498-6012
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| URL |
http://genome-www5.stanford.edu/
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| Department |
Stanford University, School of Medicine
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| Street address |
300 Pasteur Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (3) |
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| Samples (3) |
| GSM85230 |
7a (control) (red, channel 2) vs. 7b (B-naphthoflavone) (green, channel 1) |
| GSM85231 |
4a (N2 control) (red, channel 2) vs. 4b (B-naphthoflavone) (green, channel 1) |
| GSM85232 |
8a (control) (red, channel 2) vs. 8b (B-naphthoflavone) (green, channel 1) |
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| Relations |
| BioProject |
PRJNA93819 |
| Supplementary data files not provided |
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