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Series GSE36552 Query DataSets for GSE36552
Status Public on Aug 10, 2013
Title Tracing pluripotency of human early embryos and embryonic stem cells by single cell RNA-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos, and the embryonic stem cells derived from them, isolate individual cells, prepared single cell cDNAs, and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes.
Overall design We get transcriptome of 124 individual cells from human pre-implantation embryos and human embryonic stem cells by applying single cell RNA-seq technique we recently developed[1][2][3][4]. We did in-depth bioinformatic analysis to these data and found very dynamic expression of protein-coding genes.
[1] Tang, F. et al. (2010a) Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis. Cell Stem Cell 6, 468-478.
[2] Tang, F. et al. (2010b) RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat Protocols 5, 516-535.
[3] Tang, F. et al. (2009) mRNA-Seq whole-transcriptome analysis of a single cell. Nat Meth 6, 377-382.
[4] Tang, F. et al. (2011) Development and applications of single-cell transcriptome analysis. Nat Meth 8, S6-S11.
Contributor(s) Tang F, Qiao J, Li R
Citation(s) 23934149, 32968278
Submission date Mar 16, 2012
Last update date Nov 20, 2020
Contact name Mingyu Yang
Organization name Peking University
Street address 5 Yiheyuan Road
City Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (124)
GSM896803 Oocyte #1
GSM896804 Oocyte #2
GSM896805 Oocyte #3
SRA SRP011546
BioProject PRJNA153427

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36552_2-cell.bed.gz 1.8 Gb (ftp)(http) BED
GSE36552_2-cell.bed.tdf 47.9 Mb (ftp)(http) TDF
GSE36552_4-cell.bed.gz 4.4 Gb (ftp)(http) BED
GSE36552_4-cell.bed.tdf 53.9 Mb (ftp)(http) TDF
GSE36552_8-cell.bed.gz 4.7 Gb (ftp)(http) BED
GSE36552_8-cell.bed.tdf 69.1 Mb (ftp)(http) TDF
GSE36552_ESC.bed.gz 1.9 Gb (ftp)(http) BED
GSE36552_ESC.bed.tdf 37.6 Mb (ftp)(http) TDF
GSE36552_Morluae.bed.gz 3.7 Gb (ftp)(http) BED
GSE36552_Morluae.bed.tdf 59.7 Mb (ftp)(http) TDF
GSE36552_PE.bed.tdf 17.9 Mb (ftp)(http) TDF
GSE36552_PE.sort.bed.gz 1.8 Gb (ftp)(http) BED
GSE36552_RAW.tar 24.5 Gb (http)(custom) TAR (of BED, TXT)
GSE36552_TE.bed.tdf 36.6 Mb (ftp)(http) TDF
GSE36552_TE.sort.bed.gz 4.1 Gb (ftp)(http) BED
GSE36552_epi.bed.gz 1.1 Gb (ftp)(http) BED
GSE36552_epi.bed.tdf 20.5 Mb (ftp)(http) TDF
GSE36552_oocyte.bed.gz 653.3 Mb (ftp)(http) BED
GSE36552_oocyte.bed.tdf 30.3 Mb (ftp)(http) TDF
GSE36552_zygote.bed.gz 670.2 Mb (ftp)(http) BED
GSE36552_zygote.bed.tdf 33.7 Mb (ftp)(http) TDF
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Raw data are available in SRA
Processed data provided as supplementary file

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