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| Status |
Public on Jan 01, 2013 |
| Title |
Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program |
| Organism |
Ambystoma mexicanum |
| Experiment type |
Expression profiling by array
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| Summary |
Identifying the genetic program that induces limb regeneration in salamanders is an important resource for regenerative medicine, which currently lacks tools to promote regeneration of functional body structures. The genetic network underlying limb regeneration has been elusive due to the complexity of the injury response that occurs concomitant to blastema formation. Here we performed parallel expression profile time courses of non-regenerative lateral wounds versus amputated limbs in axolotl. We show that limb regeneration occurs in three distinguishable phases--early wound healing followed by a transition phase leading to establishment of the limb development program. By focusing on the transition phase, we identified 93 strictly regeneration-associated genes involved in oxidative stress response, chromatin modification, epithelial development and limb development. The specific expression of the genes was confirmed by in situ hybridization. Regeneration-specific expression databases are critical resources for understanding how regeneration-relevant phenotypes can be induced from adult cells
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| Overall design |
Regeneration of the axolotl forelimb lower arm was compared with the healing of a deep lateral injury in a high density timecourse (uncut, 3h, 6h, 9h, 12h, 24h, 36h, 52h, 72h, 120h, 168h, 288h and 528h after injury). Three independent biological replicates were performed using separate clutches of animals. Amputated and lateral wound samples were made as matched contralateral samples of four pooled animals per timepoint.
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| Contributor(s) |
Knapp D, Schulz H, Rascon CA, Volkmer M, Scholz J, Nacu E, Le M, Novozhilov S, Tazaki A, Protze S, Jacob T, Huebner N, Habermann B, Tanaka EM |
| Citation(s) |
23658691 |
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| Submission date |
Mar 12, 2012 |
| Last update date |
May 29, 2013 |
| Contact name |
Dunja Knapp |
| E-mail(s) |
dunja.knapp@crt-dresden.de
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| Phone |
+49-351-458-82035
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| Organization name |
DFG-Center for Regenerative Therapies Dresden
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| Street address |
Fetscherstraße 105
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| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL15342 |
Agilent-019788 Ambystoma mexicanum 44k_v3_20080327 [ProbeName version] |
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| Samples (80)
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| Relations |
| BioProject |
PRJNA153543 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36451_RAW.tar |
172.6 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE36451_gProcessedSignal_normalized_and_non-normalized.txt.gz |
32.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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