NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE36451 Query DataSets for GSE36451
Status Public on Jan 01, 2013
Title Comparative Transcriptional Profiling of the Axolotl Limb Identifies a Tripartite Regeneration-Specific Gene Program
Organism Ambystoma mexicanum
Experiment type Expression profiling by array
Summary Identifying the genetic program that induces limb regeneration in salamanders is an important resource for regenerative medicine, which currently lacks tools to promote regeneration of functional body structures. The genetic network underlying limb regeneration has been elusive due to the complexity of the injury response that occurs concomitant to blastema formation. Here we performed parallel expression profile time courses of non-regenerative lateral wounds versus amputated limbs in axolotl. We show that limb regeneration occurs in three distinguishable phases--early wound healing followed by a transition phase leading to establishment of the limb development program. By focusing on the transition phase, we identified 93 strictly regeneration-associated genes involved in oxidative stress response, chromatin modification, epithelial development and limb development. The specific expression of the genes was confirmed by in situ hybridization. Regeneration-specific expression databases are critical resources for understanding how regeneration-relevant phenotypes can be induced from adult cells
 
Overall design Regeneration of the axolotl forelimb lower arm was compared with the healing of a deep lateral injury in a high density timecourse (uncut, 3h, 6h, 9h, 12h, 24h, 36h, 52h, 72h, 120h, 168h, 288h and 528h after injury). Three independent biological replicates were performed using separate clutches of animals. Amputated and lateral wound samples were made as matched contralateral samples of four pooled animals per timepoint.
 
Contributor(s) Knapp D, Schulz H, Rascon CA, Volkmer M, Scholz J, Nacu E, Le M, Novozhilov S, Tazaki A, Protze S, Jacob T, Huebner N, Habermann B, Tanaka EM
Citation(s) 23658691
Submission date Mar 12, 2012
Last update date May 29, 2013
Contact name Dunja Knapp
E-mail(s) dunja.knapp@crt-dresden.de
Phone +49-351-458-82035
Organization name DFG-Center for Regenerative Therapies Dresden
Street address Fetscherstra├če 105
City Dresden
ZIP/Postal code 01307
Country Germany
 
Platforms (1)
GPL15342 Agilent-019788 Ambystoma mexicanum 44k_v3_20080327 [ProbeName version]
Samples (80)
GSM893961 limb.bud_rep1
GSM893962 mat-1_rep1
GSM893963 mat-2_rep1
Relations
BioProject PRJNA153543

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36451_RAW.tar 172.6 Mb (http)(custom) TAR (of TXT)
GSE36451_gProcessedSignal_normalized_and_non-normalized.txt.gz 32.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap