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Status |
Public on Oct 01, 2012 |
Title |
Modulation of activation-associated host cell gene expression by the apicomplexan parasite Theileria annulata |
Organisms |
Theileria annulata; Bos taurus |
Experiment type |
Expression profiling by array
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Summary |
The experiment investigates bovine gene expression in response to LPS in uninfected and Theileria annulata-infected cell cultures A subset of genes are identified which are activated in response to LPS stimulation with further modulation due to parasite infection.
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Overall design |
Six experimental conditions with three replicates per condition. Total RNA prepared from cell cultures. BL20 (uninfected bovine lymphosarcoma cell line), BL20 4 hours post-LPS stimulation, BL20 18 hours post-LPS, TBL (T. annulata infected bovine cell line), TBL 4 hours post-LPS, TBL 18 hours post-LPS. Each hydridisation represents bovine and parasite gene expression on a single channel and 2 technical replicates of each probeset are represented on the chip.
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Contributor(s) |
Durrani Z, Weir W, Pillai S, Kinnaird J, Shiels B |
Citation missing |
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Submission date |
Mar 12, 2012 |
Last update date |
Oct 02, 2012 |
Contact name |
William Weir |
Organization name |
University of Glasgow
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Department |
Institute of Infection, Immunity and Inflammation
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Lab |
Theileria lab
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Street address |
Room 220, Henry Wellcome Building, Garscube Campus, Bearsden Road
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City |
Glasgow |
ZIP/Postal code |
G61 1QH |
Country |
United Kingdom |
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Platforms (1) |
GPL15339 |
NimbleGen B. Taurus and T. annulata 385K 080829_B_taurus_JK_EXP |
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Samples (18)
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GSM893185 |
Theileria annulata infected Bos taurus TBL20 control rep1 |
GSM893186 |
Theileria annulata infected Bos taurus TBL20 control rep2 |
GSM893187 |
Theileria annulata infected Bos taurus TBL20 control rep3 |
GSM893188 |
Bos taurus BL20 LPS 4h rep1 |
GSM893189 |
Bos taurus BL20 LPS 4h rep2 |
GSM893190 |
Bos taurus BL20 LPS 4h rep3 |
GSM893191 |
Theileria annulata infected Bos taurus TBL20 LPS 4h rep1 |
GSM893192 |
Theileria annulata infected Bos taurus TBL20 LPS 4h rep2 |
GSM893193 |
Theileria annulata infected Bos taurus TBL20 LPS 4h rep3 |
GSM893194 |
Bos taurus BL20 LPS 18h rep1 |
GSM893195 |
Bos taurus BL20 LPS 18h rep2 |
GSM893196 |
Bos taurus BL20 LPS 18h rep3 |
GSM893197 |
Theileria annulata infected Bos taurus TBL20 LPS 18h rep1 |
GSM893198 |
Theileria annulata infected Bos taurus TBL20 LPS 18h rep2 |
GSM893199 |
Theileria annulata infected Bos taurus TBL20 LPS 18h rep3 |
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Relations |
BioProject |
PRJNA153233 |