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| Status |
Public on Mar 08, 2013 |
| Title |
Effect of NF-kappaB activation in bone marrow stromal cells co-cultured with CLL cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Tumor cell survival critically depends on heterotypic communication with benign cells in the microenvironment. Here we describe a novel survival signaling pathway activated in stromal cells by contact to B-cells from chronic lymphocytic leukemia (CLL) patients. The expression of PKC-βII and the subsequent activation of NF-κB in bone marrow stromal cells is a prerequisite to support the survival of malignant B-cells. PKC-β knockout mice are insusceptible to CLL-transplantations, underscoring the in vivo significance of the PKC-βII- NF-κB signaling pathway in the tumor microenvironment. Upregulated stromal PKC-βII in biopsies from CLL, breast- and pancreatic- cancer patients suggest that this pathway may commonly be activated in a variety of malignancies.
We used microarrays to determine Nemo/ IKK-gamma dependent gene expression changes in bone marrow stromal cells (BMSCs) induced by co-culturing with leukemic B-cells (CLL)
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| Overall design |
We compared mouse BMSC cells from Nemo knockout cells (Nemo+CRE) and Nemo wild type cells (Nemo-CRE) co-cultured for 5 days with leukemic B-cells microarray analysis on Affymetrix MG-430_2.0 arrays..
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| Contributor(s) |
Klein-Hitpass L, Ringshausen I |
| Citation(s) |
23328482 |
| |
| Submission date |
Mar 10, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
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| Phone |
+49 201 723 85552
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| Organization name |
Institut fuer Zellbiologie
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| Department |
Universitaetsklinikum
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| Lab |
BioChip Lab
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| Street address |
Virchowstr. 173
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| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE36416 |
Protein kinase C-beta dependent activation of NF-kB in stromal cells is indispensable for the survival of chronic lymphocytic leukemia in B-cells in vivo. |
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| Relations |
| BioProject |
PRJNA155539 |
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