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Series GSE36374 Query DataSets for GSE36374
Status Public on Mar 29, 2012
Title Histone Phosphoacetylation ChIP-seq of Kc167 cells from Drosophila
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ChIP-seq was performed using Drosophila Kc167 cells using antibodies against H3K4me3 to identify active promoters and H3K4me1 to identify active enhancers. H3K27ac ChIPseq was performed to identify active promoters and enhancers. Once enhancers and promoters were identified, JIL-1 and histone phosphorylation, H3K9acS10ph and H3K27acS28ph, ChIP-seq was performed to look at binding trends. JIL-1 and phosphoacetlation is found at low levels at inactive enhancers and shows increase at active enhancers and promoters.
 
Overall design Here we examine histone phosphorylation by JIL-1 and acetylation of H3K27ac by CBP at transcriptionally active vs. inactive promoters and enhancers. ChIP-seq is performed in Kc167 Drosophila cells using antibodies against JIL-1, H3K27acS28ph, H3K9acS10ph, H3K4me3, H3K4me1, and H3K27ac.
 
Contributor(s) Corces VG, Kellner WA, Ramos E, Van Bortle K, Takenaka N
Citation(s) 22508764, 23403565
Submission date Mar 08, 2012
Last update date May 15, 2019
Contact name Wendy A Kellner
E-mail(s) wkellne@emory.edu
Phone 404-727-4250
Organization name Emory University
Department Biology
Lab Victor Corces
Street address 1510 Clifton Road
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (2)
GPL9061 Illumina Genome Analyzer II (Drosophila melanogaster)
GPL15334 Illumina HiSeq 1000 (Drosophila melanogaster)
Samples (6)
GSM890119 H3K4me3_Kc_ChIPseq
GSM890120 H3K4me1_Kc_ChIPseq
GSM890121 H3K27ac_Kc_ChIPseq
Relations
SRA SRP011387
BioProject PRJNA153251

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Supplementary file Size Download File type/resource
GSE36374_RAW.tar 220.1 Mb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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