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Series GSE36361 Query DataSets for GSE36361
Status Public on Sep 20, 2012
Title Genome-wide maps of PRR5 binding loci in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary PRR5 transcription factor acts in the circadian clock system. To elucidate bound genes by PRR5, Chimeric protein FLAG-PRR5-GFP, was expressed under PRR5 promoter in Col-0 (PRR5pro:FLAG-PRR5-GFP/prr5). ChIP was performed using anti-GFP antibody (ab290;Abcam), which was bound to Dynabeads Protein G (100-03D;Life technologies), and ChIP DNA and input DNA were analyzed by Illumina GA II.
 
Overall design ChIP and input sequence reads were compared to find out PRR5 binding sites
 
Contributor(s) Sakakibara H, Nakamichi N
Citation(s) 23027938
Submission date Mar 08, 2012
Last update date Sep 04, 2019
Contact name Norihito Nakamichi
E-mail(s) nnakamichi@psc.riken.jp
Phone +81-52-789-4090
Fax +81-52-789-4091
Organization name RIKEN
Department Plant Science Center
Street address Suehiro1-7-22, Tsurumi
City Yokohama
ZIP/Postal code 230-0045
Country Japan
 
Platforms (1)
GPL9302 Illumina Genome Analyzer II (Arabidopsis thaliana)
Samples (2)
GSM889585 PRR5-ChIP
GSM889586 input
Relations
SRA SRP011389
BioProject PRJNA153329

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36361_ChIP-PRR5-info.txt.gz 181.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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