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Series GSE36354 Query DataSets for GSE36354
Status Public on Apr 05, 2012
Title Transcriptional Amplification in Tumor Cells with Elevated c-Myc
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Excessive expression of c-Myc occurs frequently in human cancers, where high levels are associated with tumor aggression and poor clinical outcome, but the effect of high levels of c-Myc on global gene regulation is poorly understood. We report here that in tumor cells expressing high levels of c-Myc, the transcription factor binds to E-box sequences in the core promoters of most actively transcribed genes and, unexpectedly, the enhancers of these active genes. The predominant effect of increasing c-Myc levels at both proximal and distal promoter elements is to produce higher levels of transcription at existing active genes by promoting RNA polymerase II elongation, as opposed to stimulating transcription of novel target genes. Our results argue that c-Myc overexpression drives increased transcription of growth-promoting genes, and does so by amplifying the levels of transcripts associated with the entire gene expression program of the cancer cell. Thus, excess c-Myc functions to elicit the transcriptional amplification of existing active genes through the invasion of enhancers across the cancer cell genome, thereby reducing the rate-limiting constraints required for continuous tumor growth and proliferation.
Overall design ChIP-Seq of multiple factors and histone modifications in a variety of human tumor cell lines
Contributor(s) Lin CY, Rahl PB, Loven J, Lee TI, Young RA
Citation(s) 23021215
Submission date Mar 08, 2012
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (56)
GSM894058 P493-6_T24_MYC
GSM894059 P493-6_T0_MYC
GSM894060 P493-6_T1_MYC
SRA SRP011429
BioProject PRJNA153317

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Supplementary file Size Download File type/resource
GSE36354_RAW.tar 3.7 Gb (http)(custom) TAR (of WIG, YLF)
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Raw data are available in SRA
Processed data provided as supplementary file

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