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Series GSE36258 Query DataSets for GSE36258
Status Public on Mar 08, 2012
Title Comparison of DEV infected CEF with control
Organism Gallus gallus
Experiment type Non-coding RNA profiling by array
Summary Duck enteritis virus (DEV) is an important herpesvirus pathogen of waterfowl associated with an acute, highly contagious lethal disease. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast (CEF) cultures, we determined the global changes in the microRNA (miRNA) expression profiles during DEV infection. In addition to the changes in the expression of a number of host miRNAs as a result of DEV infection, we identified several novel DEV-encoded miRNAs. Unlike most Mardivirus-encoded miRNAs, the majority of the DEV miRNAs were encoded within the unique long region of the viral genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.
 
Overall design Each microRNA is spotted on the array 6 times. We compared expression of duck enteritis virus (DEV)-infected chicken embryo fibroblasts (CEF) with CEF control.
 
Contributor(s) Yao Y, Watson M, Nair V
Citation(s) 22492913
Submission date Mar 05, 2012
Last update date Aug 03, 2012
Contact name Michael Bryan Watson
E-mail(s) mick.watson@roslin.ed.ac.uk
Phone 07990 827831
Organization name The Roslin Institute
Department ARK-Genomics
Street address Roslin Biocentre
City Roslin
State/province Midlothian, EH25 9PS
ZIP/Postal code EH25 9PS
Country United Kingdom
 
Platforms (1)
GPL15320 LC Sciences G. gallus miRChicken_17 (condensed version)
Samples (2)
GSM885594 Chicken embryo fibroblast (CEF) cells infected with duck enteritis virus (DEV)
GSM885595 Chicken embryo fibroblast (CEF) cells control
Relations
BioProject PRJNA153079

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36258_RAW.tar 250.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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